Identification of body fluid-specific DNA methylation markers for use in forensic science

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dc.contributor.authorJong Lyul Park-
dc.contributor.authorOh Hyung Kwon-
dc.contributor.authorJong-Hwan Kim-
dc.contributor.authorHyang Sook Yoo-
dc.contributor.authorH C Lee-
dc.contributor.authorK M Woo-
dc.contributor.authorSeon-Young Kim-
dc.contributor.authorS H Lee-
dc.contributor.authorYong Sung Kim-
dc.date.accessioned2017-04-19T09:55:24Z-
dc.date.available2017-04-19T09:55:24Z-
dc.date.issued2014-
dc.identifier.issn18724973-
dc.identifier.uri10.1016/j.fsigen.2014.07.011ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12116-
dc.description.abstractDNA methylation, which occurs at the 5′-position of the cytosine in CpG dinucleotides, has great potential for forensic identification of body fluids, because tissue-specific patterns of DNA methylation have been demonstrated, and DNA is less prone to degradation than proteins or RNA. Previous studies have reported several body fluid-specific DNA methylation markers, but DNA methylation differences are sometimes low in saliva and vaginal secretions. Moreover, specific DNA methylation markers in four types of body fluids (blood, saliva, semen, and vaginal secretions) have not been investigated with genome-wide profiling. Here, we investigated novel DNA methylation markers for identification of body fluids for use in forensic science using the Illumina HumanMethylation 450 K bead array, which contains over 450,000 CpG sites. Using methylome data from 16 samples of blood, saliva, semen, and vaginal secretions, we first selected 2986 hypermethylated or hypomethylated regions that were specific for each type of body fluid. We then selected eight CpG sites as novel, forensically relevant DNA methylation markers: cg06379435 and cg08792630 for blood, cg26107890 and cg20691722 for saliva, cg23521140 and cg17610929 for semen, and cg01774894 and cg14991487 for vaginal secretions. These eight selected markers were evaluated in 80 body fluid samples using pyrosequencing, and all showed high sensitivity and specificity for identification of the target body fluid. We suggest that these eight DNA methylation markers may be good candidates for developing an effective molecular assay for identification of body fluids in forensic science.-
dc.publisherElsevier-
dc.titleIdentification of body fluid-specific DNA methylation markers for use in forensic science-
dc.title.alternativeIdentification of body fluid-specific DNA methylation markers for use in forensic science-
dc.typeArticle-
dc.citation.titleForensic Science International-Genetics-
dc.citation.numberC-
dc.citation.endPage153-
dc.citation.startPage147-
dc.citation.volume13-
dc.contributor.affiliatedAuthorJong Lyul Park-
dc.contributor.affiliatedAuthorSeon-Young Kim-
dc.contributor.affiliatedAuthorYong Sung Kim-
dc.contributor.alternativeName박종열-
dc.contributor.alternativeName권오형-
dc.contributor.alternativeName김종환-
dc.contributor.alternativeName유향숙-
dc.contributor.alternativeName이한철-
dc.contributor.alternativeName우광만-
dc.contributor.alternativeName김선영-
dc.contributor.alternativeName이승환-
dc.contributor.alternativeName김용성-
dc.identifier.bibliographicCitationForensic Science International-Genetics, vol. 13, no. C, pp. 147-153-
dc.identifier.doi10.1016/j.fsigen.2014.07.011-
dc.subject.keywordAging-
dc.subject.keywordBody fluid identification-
dc.subject.keywordDNA methylation-
dc.subject.keywordEpigenetics-
dc.subject.keywordForensic-
dc.subject.localAging-
dc.subject.localBody fluid identification-
dc.subject.localDNA methylation-
dc.subject.localEpigenetics-
dc.subject.localEpigenetic-
dc.subject.localForensic-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
Korea Bioinformation Center > 1. Journal Articles
Division of Biomedical Research > Genome Editing Research Center > 1. Journal Articles
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