Production of rubusoside from stevioside by using a thermostable lactase from Thermus thermophilus and solubility enhancement of liquiritin and teniposide

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dc.contributor.authorT T H Nguyen-
dc.contributor.authorS J Jung-
dc.contributor.authorH K Kang-
dc.contributor.authorYoung-Min Kim-
dc.contributor.authorY H Moon-
dc.contributor.authorM Kim-
dc.contributor.authorD Kim-
dc.date.accessioned2017-04-19T09:55:32Z-
dc.date.available2017-04-19T09:55:32Z-
dc.date.issued2014-
dc.identifier.issn0141-0229-
dc.identifier.uri10.1016/j.enzmictec.2014.07.001ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12132-
dc.description.abstractSolubility is an important factor for achieving the desired plasma level of drug for pharmacological response. About 40% of drugs are not soluble in water in practice and therefore are slowly absorbed, which results in insufficient and uneven bioavailability and GI toxicity. Rubusoside (Ru) is a sweetener component in herbal tea and was discovered to enhance the solubility of a number of pharmaceutically and medicinally important compounds, including anticancer compounds. In this study, thirty-one hydrolyzing enzymes were screened for the conversion of stevioside (Ste) to Ru. Recombinant lactase from Thermus thermophiles which was expressed in Escherichia coli converted stevioside to rubusoside as a main product. Immobilized lactase was prepared and used for the production of rubusoside; twelve reaction cycles were repeated with 95.4% of Ste hydrolysis and 49gL-1 of Ru was produced. The optimum rubusoside synthesis yield was 86% at 200gL-1, 1200U lactase. The purified 10% rubusoside solution showed increased water solubility of liquiritin from 0.98mgmL-1 to 4.70±0.12mgmL-1 and 0mgmL-1 to 3.42±0.11mgmL-1 in the case of teniposide-
dc.publisherElsevier-
dc.titleProduction of rubusoside from stevioside by using a thermostable lactase from Thermus thermophilus and solubility enhancement of liquiritin and teniposide-
dc.title.alternativeProduction of rubusoside from stevioside by using a thermostable lactase from Thermus thermophilus and solubility enhancement of liquiritin and teniposide-
dc.typeArticle-
dc.citation.titleEnzyme and Microbial Technology-
dc.citation.number1-
dc.citation.endPage43-
dc.citation.startPage38-
dc.citation.volume64-
dc.contributor.affiliatedAuthorYoung-Min Kim-
dc.contributor.alternativeNameNguyen-
dc.contributor.alternativeName정승진-
dc.contributor.alternativeName강희경-
dc.contributor.alternativeName김영민-
dc.contributor.alternativeName문영환-
dc.contributor.alternativeName김미숙-
dc.contributor.alternativeName김도만-
dc.identifier.bibliographicCitationEnzyme and Microbial Technology, vol. 64, no. 1, pp. 38-43-
dc.identifier.doi10.1016/j.enzmictec.2014.07.001-
dc.subject.keywordImmobilized lactase-
dc.subject.keywordLiquiritin-
dc.subject.keywordRubusoside-
dc.subject.keywordTeniposide-
dc.subject.keywordThermus thermophilus-
dc.subject.localImmobilized lactase-
dc.subject.localLiquiritin-
dc.subject.localRubusoside-
dc.subject.localrubusoside-
dc.subject.localTeniposide-
dc.subject.localThermus thermophilus-
dc.description.journalClassY-
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