DC Field | Value | Language |
---|---|---|
dc.contributor.author | T T H Nguyen | - |
dc.contributor.author | S J Jung | - |
dc.contributor.author | H K Kang | - |
dc.contributor.author | Young-Min Kim | - |
dc.contributor.author | Y H Moon | - |
dc.contributor.author | M Kim | - |
dc.contributor.author | D Kim | - |
dc.date.accessioned | 2017-04-19T09:55:32Z | - |
dc.date.available | 2017-04-19T09:55:32Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 0141-0229 | - |
dc.identifier.uri | 10.1016/j.enzmictec.2014.07.001 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/12132 | - |
dc.description.abstract | Solubility is an important factor for achieving the desired plasma level of drug for pharmacological response. About 40% of drugs are not soluble in water in practice and therefore are slowly absorbed, which results in insufficient and uneven bioavailability and GI toxicity. Rubusoside (Ru) is a sweetener component in herbal tea and was discovered to enhance the solubility of a number of pharmaceutically and medicinally important compounds, including anticancer compounds. In this study, thirty-one hydrolyzing enzymes were screened for the conversion of stevioside (Ste) to Ru. Recombinant lactase from Thermus thermophiles which was expressed in Escherichia coli converted stevioside to rubusoside as a main product. Immobilized lactase was prepared and used for the production of rubusoside; twelve reaction cycles were repeated with 95.4% of Ste hydrolysis and 49gL-1 of Ru was produced. The optimum rubusoside synthesis yield was 86% at 200gL-1, 1200U lactase. The purified 10% rubusoside solution showed increased water solubility of liquiritin from 0.98mgmL-1 to 4.70±0.12mgmL-1 and 0mgmL-1 to 3.42±0.11mgmL-1 in the case of teniposide | - |
dc.publisher | Elsevier | - |
dc.title | Production of rubusoside from stevioside by using a thermostable lactase from Thermus thermophilus and solubility enhancement of liquiritin and teniposide | - |
dc.title.alternative | Production of rubusoside from stevioside by using a thermostable lactase from Thermus thermophilus and solubility enhancement of liquiritin and teniposide | - |
dc.type | Article | - |
dc.citation.title | Enzyme and Microbial Technology | - |
dc.citation.number | 1 | - |
dc.citation.endPage | 43 | - |
dc.citation.startPage | 38 | - |
dc.citation.volume | 64 | - |
dc.contributor.affiliatedAuthor | Young-Min Kim | - |
dc.contributor.alternativeName | Nguyen | - |
dc.contributor.alternativeName | 정승진 | - |
dc.contributor.alternativeName | 강희경 | - |
dc.contributor.alternativeName | 김영민 | - |
dc.contributor.alternativeName | 문영환 | - |
dc.contributor.alternativeName | 김미숙 | - |
dc.contributor.alternativeName | 김도만 | - |
dc.identifier.bibliographicCitation | Enzyme and Microbial Technology, vol. 64, no. 1, pp. 38-43 | - |
dc.identifier.doi | 10.1016/j.enzmictec.2014.07.001 | - |
dc.subject.keyword | Immobilized lactase | - |
dc.subject.keyword | Liquiritin | - |
dc.subject.keyword | Rubusoside | - |
dc.subject.keyword | Teniposide | - |
dc.subject.keyword | Thermus thermophilus | - |
dc.subject.local | Immobilized lactase | - |
dc.subject.local | Liquiritin | - |
dc.subject.local | Rubusoside | - |
dc.subject.local | rubusoside | - |
dc.subject.local | Teniposide | - |
dc.subject.local | Thermus thermophilus | - |
dc.description.journalClass | Y | - |
There are no files associated with this item.
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.