Inactivation of the phosphoglucomutase gene pgm in Paenibacillus polymyxa leads to overproduction of fusaricidin = 포스포그루코뮤타제 유전자의 불활성화에 의한 패니바실러스 폴리믹사균의 푸자리시딘 생산성 증진

Cited 7 time in scopus
Metadata Downloads
Title
Inactivation of the phosphoglucomutase gene pgm in Paenibacillus polymyxa leads to overproduction of fusaricidin = 포스포그루코뮤타제 유전자의 불활성화에 의한 패니바실러스 폴리믹사균의 푸자리시딘 생산성 증진
Author(s)
Ha Rim Kim; Soo Young Park; Seong-Bin Kim; Haeyoung JeongSoo Keun Choi; Seung-Hwan Park
Bibliographic Citation
Journal of Industrial Microbiology & Biotechnology, vol. 41, no. 9, pp. 1405-1414
Publication Year
2014
Abstract
Fusaricidin, a lipodepsipeptide isolated from Paenibacillus polymyxa, has high antimicrobial activity against fungi and Gram-positive bacteria. Through mutagenesis, we obtained two mutant strains, N1U7 and N17U7, which produce 6.2- to 7.9-fold more fusaricidin than their parent strain. Causal mutations were identified by whole-genome sequencing, and the two strains each contained at least eleven point mutations, including four common mutations. A mutation in the PPE04441 gene (pgm), encoding an α-phosphoglucomutase, was found to be an important factor in fusaricidin overproduction by complementation experiments. Null mutation of pgm in the parental strain increased fusaricidin production by 5.2-fold. Increased growth and cell viability in stationary phase, reduced exopolysaccharide production, and increased fusA expression were observed in the pgm mutant strains, which might be related to fusaricidin overproduction. This is the first report revealing that PGM deficiency leads to an overproduction of fusaricidin.
Keyword
FusaricidinPaenibacillus polymyxapgm mutationPhosphoglucomutase
ISSN
0169-4146
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10295-014-1470-z
Type
Article
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.