Metabolomics investigation of flavonoid synthesis in soybean leaves depending on the growth stage

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Title
Metabolomics investigation of flavonoid synthesis in soybean leaves depending on the growth stage
Author(s)
Hyuk-Hwan Song; Hyung Won Ryu; K J Lee; I Y Jeong; D S Kim; Sei-Ryang Oh
Bibliographic Citation
Metabolomics, vol. 10, pp. 833-841
Publication Year
2014
Abstract
Soybean (Glycine max L.) leaves have unique nutraceutical and pharmacological benefits, and have been widely used as a source of healthy and functional food stuffs in Korea. In this study, we investigated the phytochemical metabolomic changes of soybean leaves depending on growth stages (maturation period) assessed based on UPLC-QTOF-MS analysis. Principal component analysis was carried out to trace the metabolite profiles of the phytochemicals from the vegetable stage (1D) through the seven reproductive stages (R1-R7). On the loading plot, significant changes in the contents of metabolites were found during the growth, and eight flavonoid kaempferol glycosides (2, 3, 6, 8, and 10), daidzein (14), genistein (17), and coumestrol (19) were evaluated as growth markers among the 19 isolated metabolites. The kaempferol glycosides were increasingly synthesized from the 1D to the R6 stage but decreased rapidly at stages R7-R8. The extensively synthesized daidzein and genistein were shown during seed growth in the pod (R5-R6), while coumestrol was increased significantly at stages R7-R8 (maturity period). The synthetic pathway of the flavonoids could be elucidated based on the concentration of the individual metabolites. These results demonstrate that the metabolite production changed depending on the growth stage; a possible pathway could be deduced using metabolomic analysis to provide information regarding physiological characterization and optimal harvesting time for crops.
Keyword
Growth stageIsoflavonesKaempferol glyconesPterocarpansSoybean leavesUPLC-QTOF-MS
ISSN
1573-3882
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s11306-014-0640-3
Type
Article
Appears in Collections:
Ochang Branch Institute > Natural Medicine Research Center > 1. Journal Articles
Ochang Branch Institute > 1. Journal Articles
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