Induction of autophagy during in vitro maturation improves the nuclear and cytoplasmic maturation of porcine oocytes = 돼지 난소에서 핵성숙 및 세포질 성숙 동안 자기 소모

Cited 35 time in scopus
Metadata Downloads
Title
Induction of autophagy during in vitro maturation improves the nuclear and cytoplasmic maturation of porcine oocytes = 돼지 난소에서 핵성숙 및 세포질 성숙 동안 자기 소모
Author(s)
Bong Seok SongJi-Su KimYoung-Hyun KimBo Woong SimSeung-Bin Yoon; Jae Jin Cha; Seon-A Choi; Hae-Jun Yang; Seong-Eun Mun; Young-Ho ParkKang Jin JeongJae Won Huh; Sang-Rae Lee; Sang-Hyun Kim; Sun-Uk Kim; Kyu Tae Chang
Bibliographic Citation
Reproduction Fertility and Development, vol. 26, no. 7, pp. 974-981
Publication Year
2014
Abstract
While a critical role of autophagy in mammalian early embryogenesis has been demonstrated, few studies have been conducted regarding the role of autophagy in in vitro maturation (IVM) of immature oocytes. In the present study we investigated the effect of rapamycin, a chemical autophagy inducer, on the nuclear and cytoplasmic maturation of porcine oocytes. Rapamycin treatment led to increased expression of LC3-II, an autophagy marker. Compared with the control group, as well as the 5 and 10nM rapamycin treatment groups, the rate of MII oocyte production was higher in the 1nM rapamycin treatment group, indicating improvement in nuclear maturation. In the analyses of cytoplasmic maturation, we found that the level of p34cdc2, a cytoplasmic maturation marker, and the monospermic fertilisation rate were higher in the 1nM rapamycin treatment group than in the other groups. Moreover, the beneficial effect of 1nM rapamycin on cytoplasmic maturation of MII oocytes was further evidenced by increases in blastocyst formation rate, total cell number and cell survival. In the blastocyst embryos, anti-apoptotic Bcl-xL transcript levels were elevated in the 1nM rapamycin-treated group, whereas pro-apoptotic Bax transcript levels were decreased. Collectively, these results suggest that induction of autophagy during IVM contributes to enhancement of the nuclear and cytoplasmic maturation of porcine oocytes.
Keyword
apoptosisdevelopmentin vitro fertilisation
ISSN
1031-3613
Publisher
CSIRO Publishing
DOI
http://dx.doi.org/10.1071/RD13106
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Primate Resources Center > 1. Journal Articles
Ochang Branch Institute > Division of National Bio-Infrastructure > National Primate Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.