Human ChlR1 stimulates endonuclease activity of hFen1 independently of ATPase activity = ATPase 활성과 무관하게 hFen1의 endonuclase 활성을 증진시키는 인간 ChlR1

Cited 1 time in scopus
Metadata Downloads
Title
Human ChlR1 stimulates endonuclease activity of hFen1 independently of ATPase activity = ATPase 활성과 무관하게 hFen1의 endonuclase 활성을 증진시키는 인간 ChlR1
Author(s)
D H Kim; Jeong Hoon KimByoung Chul Park; D H Lee; S Cho; Sung Goo Park
Bibliographic Citation
Bulletin of Korean Chemical Society, vol. 35, no. 10, pp. 3005-3008
Publication Year
2014
Abstract
Human ChlR1 protein (hChlR1), a member of the cohesion establishment factor family, plays an important role in the segregation of sister chromatids for maintenance of genome integrity. We previously reported that hChlR1 interacts with hFen1 and stimulates its nuclease activity on the flap-structured DNA substrate covered with RPA. To elucidate the relationship between hChlR1 and Okazaki fragment processing, the effect of hChlR1 on in vitro nuclease activities of hFen1 and hDna2 was examined. Independent of ATPase activity, hChlR1 stimulated endonuclease activity of hFen1 but not that of hDna2. Our findings suggest that the acceleration of Okazaki fragment processing near cohesions may aid in reducing the size of the replication machinery, thereby facilitating its entry through the cohesin ring
Keyword
ChlR1Dna2Fen1Okazaki fragment processing
ISSN
0253-2964
Publisher
Wiley
DOI
http://dx.doi.org/10.5012/bkcs.2014.35.10.3005
Type
Article
Appears in Collections:
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.