Differential responses of three sweetpotato metallothionein genes to abiotic stress and heavy metals

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Title
Differential responses of three sweetpotato metallothionein genes to abiotic stress and heavy metals
Author(s)
Sun Ha Kim; Jae Cheol Jeong; Young Ock Ahn; Haeng Soon Lee; Sang Soo Kwak
Bibliographic Citation
Molecular Biology Reports, vol. 41, no. 10, pp. 6957-6966
Publication Year
2014
Abstract
Metallothioneins (MTs) are cysteine-rich, low molecular weight, metal-binding proteins that are widely distributed in living organisms. Plants produce metal-chelating proteins such as MTs to overcome the toxic effects of heavy metals. We cloned three MT genes from sweetpotato leaves [Ipomoea batatas (L.) Lam.]. The three IbMT genes were classified according to their cysteine residue alignment into type 1 (IbMT1), type 2 (IbMT2), and type 3 (IbMT3). IbMT1 was the most abundantly transcribed MT. It was predominantly expressed in leaves, roots, and callus. IbMT2 transcript was detected only in stems and fibrous roots, whereas IbMT3 was strongly expressed in leaves and stems. The IbMT expression profiles were investigated in plants exposed to heavy metals and abiotic stresses. The levels of IbMT1 expression were strongly elevated in response to Cd and Fe, and moderately higher in response to Cu. The IbMT3 expression pattern in response to heavy metals was similar to that of IbMT1. Exposure to abiotic stresses such as methyl viologen (MV; paraquat), NaCl, polyethylene glycol (PEG), and H2O2 up-regulated IbMT expression; IbMT1 responded strongly to MV and NaCl, whereas IbMT3 was induced by low temperature and PEG. Transgenic Escherichia coli overexpressing IbMT1 protein exhibited results suggest that IbMT could be a useful tool for engineering plants with enhanced tolerance to environmental stresses and heavy metals.
Keyword
Heavy metalMetallothioneinPhytoremediationReactive oxygen speciesSweetpotato
ISSN
0301-4851
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s11033-014-3582-y
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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