Flavonoids from Machilus japonica stems and their inhibitory effects on LDL oxidation

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Flavonoids from Machilus japonica stems and their inhibitory effects on LDL oxidation
S J Joo; H J Park; J H Park; J G Cho; Ji Hyun Kang; Tae Sook Jeong; H C Kang; D Y Lee; H S Kim; S Y Byun; N I Baek
Bibliographic Citation
International Journal of Molecular Sciences, vol. 15, no. 9, pp. 16418-16429
Publication Year
Stems of Machilus japonica were extracted with 80% aqueous methanol (MeOH) and the concentrated extract was successively extracted with ethyl acetate (EtOAc), normal butanol (n-BuOH), and water. Six flavonoids were isolated from the EtOAc fraction: (+)-taxifolin, afzelin, (?)-epicatechin, 5,3'-di-O-methyl-(?)-epicatechin, 5,7,3'-tri-O-methyl-(?)-epicatechin, and 5,7-di-O-methyl-3',4'-methylenedioxyflavan-3-ol. The chemical structures were identified using spectroscopic data including NMR, mass spectrometry and infrared spectroscopy. This is the first report of isolation of these six compounds from M. japonica. The compounds were evaluated for their diphenyl picryl hydrazinyl scavenging activity and inhibitory effects on low-density lipoprotein oxidation. Compounds 1 and 3?6 exhibited DPPH antioxidant activity equivalent with that of ascorbic acid, with half maximal inhibitory concentration (IC50) values of 0.16, 0.21, 0.17, 0.15 and 0.07 mM, respectively. The activity of compound 1 was similar to the positive control butylated hydroxytoluene, which had an IC50value of 1.9 μM, while compounds 3 and 5 showed little activity. Compounds 1, 3, and 5 exhibited LDL antioxidant activity with IC50values of 2.8, 7.1, and 4.6 μM, respectively.
Diphenyl picryl hydrazinylFlavonoidLow-density lipoprotein-oxidationMachilus japonicaNMR
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Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
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