Inhibition of the calcineurin pathway by two tannins, chebulagic acid and chebulanin, isolated from Harrisonia abyssinica Oliv. = Harrisonia abyssinica Oliv.로부터 분리한 2종류의 탄닌 화합물, chebulagic와 chebulanin에 의한 calcineurin 경로의 저해

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Title
Inhibition of the calcineurin pathway by two tannins, chebulagic acid and chebulanin, isolated from Harrisonia abyssinica Oliv. = Harrisonia abyssinica Oliv.로부터 분리한 2종류의 탄닌 화합물, chebulagic와 chebulanin에 의한 calcineurin 경로의 저해
Author(s)
Won Jeong Lee; Jae Sun Moon; S I Kim; Young Tae Kim; O Nash; Y S Bahn; Sung Uk Kim
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 24, no. 10, pp. 1377-1381
Publication Year
2014
Abstract
In order to discover and develop novel signaling inhibitors from plants, a screening system was established targeting the two-component system of Cryptococcus neoformans by using the wild type and a calcineurin mutant of C. neoformans, based on the counter-regulatory action of high-osmolarity glycerol (Hog1) mitogen-activated protein kinase and the calcineurin pathways in C. neoformans. Among 10,000 plant extracts, that from Harrisonia abyssinica Oliv. exhibited the most potent inhibitory activity against C. neoformans var. grubii H99 with fludioxonil. Bioassay-guided fractionation was used to isolate two bioactive compounds from H. abyssinica, and these compounds were identified as chebulagic acid and chebulanin using spectroscopic methods. These compounds specifically inhibited the calcineurin pathway in C. neoformans. Moreover, they exhibited potent antifungal activities against various human pathogenic fungi with minimum inhibitory concentrations ranging from 0.25 to over 64 μg/ml.
Keyword
Signaling pathwayCryptococcus neoformansTwo-component systemCalcineurin inhibitorAntifungal activity
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.4014/jmb.1405.05030
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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