Role of NADH: quinone oxidoreductase-1 in the tight junctions of colonic epithelial cells

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Title
Role of NADH: quinone oxidoreductase-1 in the tight junctions of colonic epithelial cells
Author(s)
S T Nam; Jung Hwan Hwang; D H Kim; M J Park; I H Lee; H J Nam; J K Kang; S K Kim; J S Hwang; H K Chung; M Shong; Chul Ho Lee; H Kim
Bibliographic Citation
BMB Reports, vol. 47, no. 9, pp. 494-499
Publication Year
2014
Abstract
NADH:quinone oxidoreductase 1 (NQO1) is known to be involved in the regulation of energy synthesis and metabolism, and the functional studies of NQO1 have largely focused on metabolic disorders. Here, we show for the first time that compared to NQO1-WT mice, NQO1-KO mice exhibited a marked increase of permeability and spontaneous inflammation in the gut. In the DSS-induced colitis model, NQO1-KO mice showed more severe inflammatory responses than NQO1-WT mice. Interestingly, the transcript levels of claudin and occludin, the major tight junction molecules of gut epithelial cells, were significantly decreased in NQO1-KO mice. The colons of NQO1-KO mice also showed high levels of reactive oxygen species (ROS) and histone deacetylase (HDAC) activity, which are known to affect transcriptional regulation. Taken together, these novel findings indicate that NQO1 contributes to the barrier function of gut epithelial cells by regulating the transcription of tight junction molecules.
Keyword
Barrier dysfunction of epithelial cellsChromosome condensationClaudin-1Gut epithelial cell tight junctionGut inflammationHistone acetylation/deacetylationNQO1 knockout miceOccludinTranscription
ISSN
1225-8687
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.5483/BMBRep.2014.47.9.196
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > Laboratory Animal Resource & Research Center > 1. Journal Articles
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