DC Field | Value | Language |
---|---|---|
dc.contributor.author | T W Chung | - |
dc.contributor.author | S J Kim | - |
dc.contributor.author | H J Choi | - |
dc.contributor.author | K H Song | - |
dc.contributor.author | U H Jin | - |
dc.contributor.author | Dae Yeul Yu | - |
dc.contributor.author | J K Seong | - |
dc.contributor.author | J G Kim | - |
dc.contributor.author | K J Kim | - |
dc.contributor.author | Jeong Heon Ko | - |
dc.contributor.author | K T Ha | - |
dc.contributor.author | Y C Lee | - |
dc.contributor.author | C H Kim | - |
dc.date.accessioned | 2017-04-19T09:56:48Z | - |
dc.date.available | 2017-04-19T09:56:48Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 1476-4598 | - |
dc.identifier.uri | 10.1186/1476-4598-13-222 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/12216 | - |
dc.description.abstract | Background: The metastasis of hematogenous cancer cells is associated with abnormal glycosylation such as sialyl lewis antigens. Although the hepatitis B virus X protein (HBx) plays important role in liver disease, the precise function of HBx on aberrant glycosylation for metastasis remains unclear.Methods: The human hepatocellular carcinoma tissues, HBx transgenic mice and HBx-transfected cells were used to check the correlation of expressions between HBx and Sialyl lewis antigen for cancer metastasis. To investigate whether expression levels of glycosyltransferases induced in HBx-transfected cells are specifically associated with sialyl lewis A (SLA) synthesis, which enhances metastasis by interaction of liver cancer cells with endothelial cells, ShRNA and siRNAs targeting specific glycosyltransferases were used.Results: HBx expression in liver cancer region of HCC is associated with the specific synthesis of SLA. Furthermore, the SLA was specifically induced both in liver tissues from HBx-transgenic mice and in in vitro HBx-transfected cells. HBx increased transcription levels and activities of α2-3 sialyltransferases (ST3Gal III), α1-3/4 fucosyltransferases III and VII (FUT III and VII) genes, which were specific for SLA synthesis, allowing dramatic cell-cell adhesion for metastatic potential. Interestingly, HBx specifically induced expression of N-acetylglucosamine-β1-3 galactosyltransferase V (β1-3GalT 5) gene associated with the initial synthesis of sialyl lewis A, but not β1-4GalT I. The β1-3GalT 5 shRNA suppressed SLA expression by HBx, blocking the adhesion of HBx-transfected cells to the endothelial cells. Moreover, β1-3GalT 5 silencing suppressed lung metastasis of HBx-transfected cells in in vivo lung metastasis system.Conclusion: HBx targets the specific glycosyltransferases for the SLA synthesis and this process regulates hematogenous cancer cell adhesion to endothelial cells for cancer metastasis. | - |
dc.publisher | Springer-BMC | - |
dc.title | Hepatitis B virus X protein specially regulates the sialyl lewis a synthesis among glycosylation events for metastasis = 암전이 당질화 과정에서 시알릴 루이스 a 합성을 조절하는 B형 간염바이러스의 X 단백질 | - |
dc.title.alternative | Hepatitis B virus X protein specially regulates the sialyl lewis a synthesis among glycosylation events for metastasis | - |
dc.type | Article | - |
dc.citation.title | Molecular Cancer | - |
dc.citation.number | 1 | - |
dc.citation.endPage | 222 | - |
dc.citation.startPage | 222 | - |
dc.citation.volume | 13 | - |
dc.contributor.affiliatedAuthor | Dae Yeul Yu | - |
dc.contributor.affiliatedAuthor | Jeong Heon Ko | - |
dc.contributor.alternativeName | 정태욱 | - |
dc.contributor.alternativeName | 김석조 | - |
dc.contributor.alternativeName | 최희정 | - |
dc.contributor.alternativeName | 송권호 | - |
dc.contributor.alternativeName | 진운호 | - |
dc.contributor.alternativeName | 유대열 | - |
dc.contributor.alternativeName | 성제경 | - |
dc.contributor.alternativeName | 김종국 | - |
dc.contributor.alternativeName | 김극준 | - |
dc.contributor.alternativeName | 고정헌 | - |
dc.contributor.alternativeName | 하기태 | - |
dc.contributor.alternativeName | 이영춘 | - |
dc.contributor.alternativeName | 김철호 | - |
dc.identifier.bibliographicCitation | Molecular Cancer, vol. 13, no. 1, pp. 222-222 | - |
dc.identifier.doi | 10.1186/1476-4598-13-222 | - |
dc.subject.keyword | E-selectin | - |
dc.subject.keyword | Endothelial cells | - |
dc.subject.keyword | Hepatitis B virus | - |
dc.subject.keyword | Hepatocellular carcinoma | - |
dc.subject.keyword | Sialyl lewis antigen | - |
dc.subject.local | E-selectin | - |
dc.subject.local | e-selectin | - |
dc.subject.local | Endothelial cell | - |
dc.subject.local | endothelial cells | - |
dc.subject.local | endothelial cell | - |
dc.subject.local | Endothelial cells | - |
dc.subject.local | hepatitis B virus (HBV) | - |
dc.subject.local | Hepatitis B Virus | - |
dc.subject.local | Hepatitis B virus (HBV) | - |
dc.subject.local | hepatitis B virus | - |
dc.subject.local | hepatitis B Virus (HBV) | - |
dc.subject.local | Hepatitis B virus | - |
dc.subject.local | Hepatocellular carcinomas | - |
dc.subject.local | Hepatocellular carcinoma (HCC) | - |
dc.subject.local | Hepatocellular carcinoma | - |
dc.subject.local | hepatocellular carcinoma (HCC) | - |
dc.subject.local | hepatocellular carcinoma | - |
dc.subject.local | Sialyl lewis antigen | - |
dc.description.journalClass | Y | - |
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