Hepatitis B virus X protein specially regulates the sialyl lewis a synthesis among glycosylation events for metastasis = 암전이 당질화 과정에서 시알릴 루이스 a 합성을 조절하는 B형 간염바이러스의 X 단백질

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dc.contributor.authorT W Chung-
dc.contributor.authorS J Kim-
dc.contributor.authorH J Choi-
dc.contributor.authorK H Song-
dc.contributor.authorU H Jin-
dc.contributor.authorDae Yeul Yu-
dc.contributor.authorJ K Seong-
dc.contributor.authorJ G Kim-
dc.contributor.authorK J Kim-
dc.contributor.authorJeong Heon Ko-
dc.contributor.authorK T Ha-
dc.contributor.authorY C Lee-
dc.contributor.authorC H Kim-
dc.date.accessioned2017-04-19T09:56:48Z-
dc.date.available2017-04-19T09:56:48Z-
dc.date.issued2014-
dc.identifier.issn1476-4598-
dc.identifier.uri10.1186/1476-4598-13-222ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12216-
dc.description.abstractBackground: The metastasis of hematogenous cancer cells is associated with abnormal glycosylation such as sialyl lewis antigens. Although the hepatitis B virus X protein (HBx) plays important role in liver disease, the precise function of HBx on aberrant glycosylation for metastasis remains unclear.Methods: The human hepatocellular carcinoma tissues, HBx transgenic mice and HBx-transfected cells were used to check the correlation of expressions between HBx and Sialyl lewis antigen for cancer metastasis. To investigate whether expression levels of glycosyltransferases induced in HBx-transfected cells are specifically associated with sialyl lewis A (SLA) synthesis, which enhances metastasis by interaction of liver cancer cells with endothelial cells, ShRNA and siRNAs targeting specific glycosyltransferases were used.Results: HBx expression in liver cancer region of HCC is associated with the specific synthesis of SLA. Furthermore, the SLA was specifically induced both in liver tissues from HBx-transgenic mice and in in vitro HBx-transfected cells. HBx increased transcription levels and activities of α2-3 sialyltransferases (ST3Gal III), α1-3/4 fucosyltransferases III and VII (FUT III and VII) genes, which were specific for SLA synthesis, allowing dramatic cell-cell adhesion for metastatic potential. Interestingly, HBx specifically induced expression of N-acetylglucosamine-β1-3 galactosyltransferase V (β1-3GalT 5) gene associated with the initial synthesis of sialyl lewis A, but not β1-4GalT I. The β1-3GalT 5 shRNA suppressed SLA expression by HBx, blocking the adhesion of HBx-transfected cells to the endothelial cells. Moreover, β1-3GalT 5 silencing suppressed lung metastasis of HBx-transfected cells in in vivo lung metastasis system.Conclusion: HBx targets the specific glycosyltransferases for the SLA synthesis and this process regulates hematogenous cancer cell adhesion to endothelial cells for cancer metastasis.-
dc.publisherSpringer-BMC-
dc.titleHepatitis B virus X protein specially regulates the sialyl lewis a synthesis among glycosylation events for metastasis = 암전이 당질화 과정에서 시알릴 루이스 a 합성을 조절하는 B형 간염바이러스의 X 단백질-
dc.title.alternativeHepatitis B virus X protein specially regulates the sialyl lewis a synthesis among glycosylation events for metastasis-
dc.typeArticle-
dc.citation.titleMolecular Cancer-
dc.citation.number1-
dc.citation.endPage222-
dc.citation.startPage222-
dc.citation.volume13-
dc.contributor.affiliatedAuthorDae Yeul Yu-
dc.contributor.affiliatedAuthorJeong Heon Ko-
dc.contributor.alternativeName정태욱-
dc.contributor.alternativeName김석조-
dc.contributor.alternativeName최희정-
dc.contributor.alternativeName송권호-
dc.contributor.alternativeName진운호-
dc.contributor.alternativeName유대열-
dc.contributor.alternativeName성제경-
dc.contributor.alternativeName김종국-
dc.contributor.alternativeName김극준-
dc.contributor.alternativeName고정헌-
dc.contributor.alternativeName하기태-
dc.contributor.alternativeName이영춘-
dc.contributor.alternativeName김철호-
dc.identifier.bibliographicCitationMolecular Cancer, vol. 13, no. 1, pp. 222-222-
dc.identifier.doi10.1186/1476-4598-13-222-
dc.subject.keywordE-selectin-
dc.subject.keywordEndothelial cells-
dc.subject.keywordHepatitis B virus-
dc.subject.keywordHepatocellular carcinoma-
dc.subject.keywordSialyl lewis antigen-
dc.subject.localE-selectin-
dc.subject.locale-selectin-
dc.subject.localEndothelial cell-
dc.subject.localendothelial cells-
dc.subject.localendothelial cell-
dc.subject.localEndothelial cells-
dc.subject.localhepatitis B virus (HBV)-
dc.subject.localHepatitis B Virus-
dc.subject.localHepatitis B virus (HBV)-
dc.subject.localhepatitis B virus-
dc.subject.localhepatitis B Virus (HBV)-
dc.subject.localHepatitis B virus-
dc.subject.localHepatocellular carcinomas-
dc.subject.localHepatocellular carcinoma (HCC)-
dc.subject.localHepatocellular carcinoma-
dc.subject.localhepatocellular carcinoma (HCC)-
dc.subject.localhepatocellular carcinoma-
dc.subject.localSialyl lewis antigen-
dc.description.journalClassY-
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Synthetic Biology and Bioengineering Research Institute > Genome Editing Research Center > 1. Journal Articles
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