Purification and functional characterization of the first stilbene glucoside-specific beta-glucosidase isolated from Lactobacillus kimchi = 락토바실러스 유래 균으로부터 새로운 스틸벤 배당화합물의 분리 및 기능성 연구
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- Title
- Purification and functional characterization of the first stilbene glucoside-specific beta-glucosidase isolated from Lactobacillus kimchi = 락토바실러스 유래 균으로부터 새로운 스틸벤 배당화합물의 분리 및 기능성 연구
- Author(s)
- Jin-A Ko; Ji Young Park; Hyung Jun Kwon; Young Bae Ryu; Hyung Jae Jeong; Su-Jin Park; Cha Young Kim; Hyun-Mee Oh; Chan Sun Park; Y H Lim; D Kim; Mun Chual Rho; Woo Song Lee; Young-Min Kim
- Bibliographic Citation
- Enzyme and Microbial Technology, vol. 67, pp. 59-66
- Publication Year
- 2014
- Abstract
- This study aimed to develop viable enzymes for bioconversion of resveratrol-glucoside into resveratrol. Out of 13 bacterial strains tested, Lactobacillus kimchi JB301 could completely convert polydatin into resveratrol. The purified enzyme had an optimum temperature of 30-40°C and optimum pH of pH 5.0 against polydatin. This enzyme showed high substrate specificities towards different substrates in the following order: isorhaponticin "polydatin" mulberroside A>oxyresveratrol-3-O-glucoside. Additionally, it rarely hydrolyzed astringin and desoxyrhaponticin. Based on these catalytic specificities, we suggest this enzyme be named stilbene glucoside-specific β-glucosidase. Furthermore, polydatin extracts from Polygonum cuspidatum were successfully converted to resveratrol with a high yield (of over 99%). Stilbene glucoside-specific β-glucosidase is the first enzyme isolated from lactic acid bacteria capable of bio-converting various stilbene glucosides into stilbene.
- Keyword
- BioconversionLactobacillus kimchiPolydatinResveratrolβ-Glucosidase
- ISSN
- 0141-0229
- Publisher
- Elsevier
- DOI
- http://dx.doi.org/10.1016/j.enzmictec.2014.09.001
- Type
- Article
- Appears in Collections:
- Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
Jeonbuk Branch Institute > 1. Journal Articles
- Files in This Item:
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