Overexpression of codA gene confers enhanced tolerance to abiotic stresses in alfalfa

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Title
Overexpression of codA gene confers enhanced tolerance to abiotic stresses in alfalfa
Author(s)
H Li; Z Wang; Q Ke; Chang Yoon Ji; Jae Cheol Jeong; Haeng Soon Lee; Y P Lim; B Xu; X P Deng; Sang Soo Kwak
Bibliographic Citation
Plant Physiology and Biochemistry, vol. 85, pp. 31-40
Publication Year
2014
Abstract
We generated transgenic alfalfa plants (. Medicago sativa L. cv. Xinjiang Daye) expressing a bacterial codA gene in chloroplasts under the control of the SWPA2 promoter (referred to as SC plants) and evaluated the plants under various abiotic stress conditions. Three transgenic plants (SC7, SC8, and SC9) were selected for further characterization based on the strong expression levels of codA in response to methyl viologen (MV)-mediated oxidative stress. SC plants showed enhanced tolerance to NaCl and drought stress on the whole plant level due to induced expression of codA. When plants were subjected to 250mM NaCl treatment for 2 weeks, SC7 and SC8 plants maintained higher chlorophyll contents and lower malondialdehyde levels than non-transgenic (NT) plants. Under drought stress conditions, all SC plants showed enhanced tolerance to drought stress through maintaining high relative water contents and increased levels of glycinebetaine and proline compared to NT plants. Under normal conditions, SC plants exhibited increased growth due to increased expression of auxin-related IAA genes compared to NT plants. These results suggest that the SC plants generated in this study will be useful for enhanced biomass production on global marginal lands, such as high salinity and arid lands, yielding a sustainable agricultural product.
Keyword
AlfalfaBiomassCodACodADrought stressGBGlycinebetaineIAAMDAMSMVNTOxidative stressPCRQ-RT-PCRROSRWCSalt stressSWAP2 promoterSWPA2T-nosTBATCA
ISSN
0981-9428
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.plaphy.2014.10.010
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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