Dephosphorylation of CCAAT/enhancer-binding protein β by protein phosphatase 2A containing B56δ is required at the early time of adipogenesis

Cited 4 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorM Park-
dc.contributor.authorY A Choi-
dc.contributor.authorHee Gu Lee-
dc.contributor.authorK I Kim-
dc.contributor.authorJ S Lim-
dc.contributor.authorM S Lee-
dc.contributor.authorK S Oh-
dc.contributor.authorY Yang-
dc.date.accessioned2017-04-19T09:57:28Z-
dc.date.available2017-04-19T09:57:28Z-
dc.date.issued2014-
dc.identifier.issn1388-1981-
dc.identifier.uri10.1016/j.bbalip.2014.08.008ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12229-
dc.description.abstractIt is known that protein phosphatase 2A (PP2A) expression is increased in high-fat diet (HFD)-induced obese mice, but the role of PP2A in adipogenesis as well as obesity remains to be addressed. In this study, the role of PP2A in adipogenesis was explored. Preadipocytes were treated with okadaic acid (OA) during adipogenesis and the degree of adipogenesis was determined. The OA treatment blocked adipogenesis at the early time of adipogenesis, but not at the late time. In the early time of adipogenesis, CCAAT/enhancer-binding protein β (C/EBPβ) activation is preceded by the expression of key adipogenic transcription factors including PPARγ and C/EBPα, which function at the late time of adipogenesis, and then C/EBPβ is degraded. However, the inhibition of PP2A by OA treatment sustained phosphorylation of C/EBPβ and delayed its degradation. In turn, PPARγ and C/EBPα activation was altered. Among the various regulatory B56 subunits consisting of PP2A holoenzyme, B56δ was directly bound to C/EBPβ and was responsible for the dephosphorylation of C/EBPβ by PP2A. Taken together, these findings suggest that the phosphorylation of C/EBPβ after hormonal induction has to be inactivated by PP2A containing B56δ at the early time of adipogenesis to allow the completion of adipogenesis.-
dc.publisherElsevier-
dc.titleDephosphorylation of CCAAT/enhancer-binding protein β by protein phosphatase 2A containing B56δ is required at the early time of adipogenesis-
dc.title.alternativeDephosphorylation of CCAAT/enhancer-binding protein β by protein phosphatase 2A containing B56δ is required at the early time of adipogenesis-
dc.typeArticle-
dc.citation.titleBiochimica et Biophysica Acta-Molecular and Cell Biology of Lipids-
dc.citation.number11-
dc.citation.endPage1618-
dc.citation.startPage1608-
dc.citation.volume1841-
dc.contributor.affiliatedAuthorHee Gu Lee-
dc.contributor.alternativeName박미영-
dc.contributor.alternativeName최연아-
dc.contributor.alternativeName이희구-
dc.contributor.alternativeName김근일-
dc.contributor.alternativeName임종석-
dc.contributor.alternativeName이명석-
dc.contributor.alternativeName오기숙-
dc.contributor.alternativeName양영-
dc.identifier.bibliographicCitationBiochimica et Biophysica Acta-Molecular and Cell Biology of Lipids, vol. 1841, no. 11, pp. 1608-1618-
dc.identifier.doi10.1016/j.bbalip.2014.08.008-
dc.subject.keywordAdipogenesis-
dc.subject.keywordB56δ-
dc.subject.keywordC/EBPβ-
dc.subject.keywordExtracellular signal regulated kinase (ERK)-
dc.subject.keywordProtein phosphatase 2A (PP2A)-
dc.subject.localadipogenesis-
dc.subject.localADIPOGENESIS-
dc.subject.localAdipogenesis-
dc.subject.localB56δ-
dc.subject.localC/EBPβ-
dc.subject.localC/EBP-beta-
dc.subject.localextracellular signal-regulated kinase-
dc.subject.localExtracellular Signal-Regulated Kinase-
dc.subject.localExtracellular signal regulated kinase (ERK)-
dc.subject.localExtracellular signal-regulated kinase-
dc.subject.localProtein phosphatase 2A (PP2A)-
dc.subject.localProtein phosphatase 2A-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.