Proteomic characterization of the outer membrane vesicle of Pseudomonas putida KT2440

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dc.contributor.authorC W Choi-
dc.contributor.authorE C Park-
dc.contributor.authorS H Yun-
dc.contributor.authorS Y Lee-
dc.contributor.authorY G Lee-
dc.contributor.authorY Hong-
dc.contributor.authorK R Park-
dc.contributor.authorSang-Hyun Kim-
dc.contributor.authorG H Kim-
dc.contributor.authorS I Kim-
dc.date.accessioned2017-04-19T09:57:42Z-
dc.date.available2017-04-19T09:57:42Z-
dc.date.issued2014-
dc.identifier.issn1535-3893-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12236-
dc.description.abstractOuter membrane vesicles (OMVs) are produced by various pathogenic Gram-negative bacteria such as Escherichia coli, Pseudomonas aeruginosa, and Acinetobacter baumannii. In this study, we isolated OMVs from a representative soil bacterium, Pseudomonas putida KT2440, which has a biodegradative activity toward various aromatic compounds. Proteomic analysis identified the outer membrane proteins (OMPs) OprC, OprD, OprE, OprF, OprH, OprG, and OprW as major components of the OMV of P. putida KT2440. The production of OMVs was dependent on the nutrient availability in the culture media, and the up- or down-regulation of specific OMPs was observed according to the culture conditions. In particular, porins (e.g., benzoate-specific porin, BenF-like porin) and enzymes (e.g., catechol 1,2-dioxygenase, benzoate dioxygenase) for benzoate degradation were uniquely found in OMVs prepared from P. putida KT2440 that were cultured in media containing benzoate as the energy source. OMVs of P. putida KT2440 showed low pathological activity toward cultured cells that originated from human lung cells, which suggests their potential as adjuvants or OMV vaccine carriers. Our results suggest that the protein composition of the OMVs of P. putida KT2440 reflects the characteristics of the total proteome of P. putida KT2440.-
dc.publisherAmer Chem Soc-
dc.titleProteomic characterization of the outer membrane vesicle of Pseudomonas putida KT2440-
dc.title.alternativeProteomic characterization of the outer membrane vesicle of Pseudomonas putida KT2440-
dc.typeArticle-
dc.citation.titleJournal of Proteome Research-
dc.citation.number10-
dc.citation.endPage4309-
dc.citation.startPage4298-
dc.citation.volume13-
dc.contributor.affiliatedAuthorSang-Hyun Kim-
dc.contributor.alternativeName최치원-
dc.contributor.alternativeName박창균-
dc.contributor.alternativeName윤성호-
dc.contributor.alternativeName이상엽-
dc.contributor.alternativeName이열균-
dc.contributor.alternativeName홍연희-
dc.contributor.alternativeName박경량-
dc.contributor.alternativeName김상현-
dc.contributor.alternativeName김건화-
dc.contributor.alternativeName김승일-
dc.identifier.bibliographicCitationJournal of Proteome Research, vol. 13, no. 10, pp. 4298-4309-
dc.identifier.doi10.1021/pr500411d-
dc.subject.keywordmass spectrometry-
dc.subject.keywordouter membrane vesicle-
dc.subject.keywordPseudomonas putida KT2440-
dc.subject.localMass spetrometry-
dc.subject.localMass spectrometry (MS)-
dc.subject.localmass spectrometry-
dc.subject.localMass spectrometry-
dc.subject.localmass spectrometry (MS)-
dc.subject.localouter membrane vesicle-
dc.subject.localOuter membrane vesicle-
dc.subject.localOuter membrane vesicles-
dc.subject.localPseudomonas putida KT2440-
dc.description.journalClassY-
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