Identification of DNA aptamers toward epithelial cell adhesion molecule via Cell-SELEX

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Title
Identification of DNA aptamers toward epithelial cell adhesion molecule via Cell-SELEX
Author(s)
Ji Won Kim; Eun Young Kim; Sun Young Kim; Sang Kyung Byun; Dasom Lee; Kyoung Jin OhWon Kon KimBaek Soo HanSeung-Wook ChiSang Chul LeeKwang-Hee Bae
Bibliographic Citation
Molecules and Cells, vol. 37, no. 10, pp. 742-746
Publication Year
2014
Abstract
The epithelial cell adhesion molecule (EpCAM, also known as CD326) is a transmembrane glycoprotein that is specifically detected in most adenocarcinomas and cancer stem cells. In this study, we performed a Cell systematic evolution of ligands by exponential enrichment (SELEX) experiment to isolate the aptamers against EpCAM. After seven round of Cell SELEX, we identified several aptamer candidates. Among the selected aptamers, EP166 specifically binds to cells expressing EpCAM with an equilibrium dissociation constant (Kd) in a micromolar range. On the other hand, it did not bind to negative control cells. Moreover, EP166 binds to J1ES cells, a mouse embryonic stem cell line. Therefore, the isolated aptamers against EpCAM could be used as a stem cell marker or in other applications in both stem cell and cancer studies.
Keyword
aptamerEpCAMSELEXstem cell marker
ISSN
1016-8478
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.14348/molcells.2014.0208
Type
Article
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Division of Research on National Challenges > Biodefense Research Center > 1. Journal Articles
Division of Biomedical Research > 1. Journal Articles
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