Tussilago farfara L. augments TRAIL-induced apoptosis through MKK7/JNK activation by inhibition of MKK7-TIPRL in human hepatocellular carcinoma cells = 간암세포주 Tussilago farfara L. 추출물 MKK7-TIPRL의 결합저해와 MKK7/JNK activation의 TRAIL유도 세포사멸

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Title
Tussilago farfara L. augments TRAIL-induced apoptosis through MKK7/JNK activation by inhibition of MKK7-TIPRL in human hepatocellular carcinoma cells = 간암세포주 Tussilago farfara L. 추출물 MKK7-TIPRL의 결합저해와 MKK7/JNK activation의 TRAIL유도 세포사멸
Author(s)
Hyo Jung Lee; Hyun Soo Cho; Soo Young Jun; Jeong Ju LeeJi Yong Yoon; Jae Hye Lee; Hyuk-Hwan Song; Sangho ChoiSoo Yong Kim; V Saloura; C G Park; Nam-Soon Kim
Bibliographic Citation
Oncology Reports, vol. 32, no. 3, pp. 1117-1123
Publication Year
2014
Abstract
Induction of apoptosis through activation of the TRAIL pathway is considered to be a promising anticancer strategy due to its ability to selectively induce apoptosis in cancer cells. However, the ability of cancer cells to acquire TRAIL resistance has limited the clinical translation of this approach. We previously reported that the TOR signaling pathway regulator-like (TIPRL) protein contributes to the resistance to TRAIL-induced apoptosis by inhibiting the MKK7-c-Jun N-terminal kinase (JNK) pathway via MKK7?TIPRL interaction. In the present study, we identified Tussilago farfara L. (TF) as a novel TRAIL sensitizer among 500 natural products using an ELISA system that specifically detects the MKK7-TIPRL interaction, and we validated candidates by GST-pull down assay. Co-treatment of Huh7 cells with TF and TRAIL induced apoptosis via inhibition of the MKK7-TIPRL interaction and an increase in MKK7/JNK phosphorylation. This is the first report to describe TF as a novel TRAIL sensitizer, unveiling a potentially novel therapeutic strategy in cancer therapy
Keyword
ApoptosisHCCMKK7TIPRLTRAIL resistanceTussilago farfara L.
ISSN
1021-335X
Publisher
Spandidos Publ Ltd
DOI
http://dx.doi.org/10.3892/or.2014.3279
Type
Article
Appears in Collections:
Division of Research on National Challenges > Stem Cell Convergenece Research Center > 1. Journal Articles
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > International Biological Material Research Center > 1. Journal Articles
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