Characterization of a lichenase isolated from soil metagenome = 메타게놈유래 리치네이즈

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Title
Characterization of a lichenase isolated from soil metagenome = 메타게놈유래 리치네이즈
Author(s)
Sang Yoon Kim; Doo-Byoung OhOh Suk Kwon
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 24, no. 12, pp. 1699-1706
Publication Year
2014
Abstract
A lichenase gene (mt-lic) was identified for the first time through function-based screening of a soil metagenomic library. Its deduced amino acid sequence exhibited a high degree of homology with endo-β-1,3-1,4-glucanase having both lichenase and chitosanase activities, encoded by the bgc gene of Bacillus circulans WL-12. The recombinant lichenase overexpressed and purified from Escherichia coli was able to efficiently hydrolyze both barley β-glucan and lichenan. The enzyme showed maximal activity at a pH of 6.0 at 50°C, with Azo-barley-glucan as the substrate. The metal ions Mn2+, Mg2+, Ca2+, and Fe2+ enhanced the enzymatic activity, whereas the Cu2+ and Zn2+ ions inhibited the enzymatic activity. The Km and Vmax values of the purified lichenase were determined to be 0.45 mg/ml and 24.83 U/min per mg of protein, respectively
Keyword
Endo-β-1,3-1,4-glucanaseGlycosyl hydrolase family 8LichenaseRecombinant proteinSoil metagenome
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.4014/jmb.1406.06012
Type
Article
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
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