Expression and characterization of a novel 2-deoxyribose-5-phosphate aldolase from Haemophilus influenzae Rd KW20

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dc.contributor.authorMi-Hee Woo-
dc.contributor.authorMin Soo Kim-
dc.contributor.authorN Chung-
dc.contributor.authorJoong Su Kim-
dc.description.abstractA codon-optimized 2-deoxyribose-5-phosphate aldolase (DERA) gene from Haemophilus influenzae Rd KW20 was synthesized and expressed in Escherichia coli, and the biochemical properties of its product were investigated. DERA was purified using affinity chromatography and characterized using 2-deoxyribose-5-phosphate as the substrate. Specific activity of the recombinant DERA was 34.1 Umg -1. The pH and temperature optima were 7.5 and 40°C, respectively. Additionally, the recombinant enzyme retained stability up to temperatures below 50°C. Maximal enzyme activity was observed in presence of 300 mM of acetaldehyde. The apparent Km and Vmax of purified enzyme towards 2-deoxyribose-5-phosphate were 0.14 mM and 70.42 μmol min -1 mg -1 and towards 2-deoxy-D-ribose were 24.77 mM and 1.94 μmol min -1 mg -1, respectively. For synthesis of statin intermediates, the bioconversion process for production of (3R, 5S)-6-chloro-2,4,6-trideoxy-erythro-hexose from chloroacetaldehyde and acetaldehyde using the recombinant DERA was studied and this process took 3 h for maximal conversion. This recombinant DERA could be potentially applied in the production of (3R, 5S)-6-chloro-2,4,6-trideoxy-erythro-hexose.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleExpression and characterization of a novel 2-deoxyribose-5-phosphate aldolase from Haemophilus influenzae Rd KW20-
dc.title.alternativeExpression and characterization of a novel 2-deoxyribose-5-phosphate aldolase from Haemophilus influenzae Rd KW20-
dc.citation.titleJournal of Korean Society for Applied Biological Chemistry-
dc.contributor.affiliatedAuthorMi-Hee Woo-
dc.contributor.affiliatedAuthorMin Soo Kim-
dc.contributor.affiliatedAuthorJoong Su Kim-
dc.identifier.bibliographicCitationJournal of Korean Society for Applied Biological Chemistry, vol. 57, no. 5, pp. 655-660-
dc.subject.keyword2-deoxyribose-5-phosphate aldolase-
dc.subject.keywordHaemophilus influenzae-
dc.subject.keywordstatin intermediates synthesis-
dc.subject.local2-deoxyribose-5-phosphate aldolase-
dc.subject.localHaemophilus influenzae-
dc.subject.localstatin intermediates synthesis-
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
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