Aneurinibacillus soli sp. nov., isolated from mountain soil

Abstract

A novel bacterial strain designated CB4Twas isolated from soil from the Hallasan, Jeju, Korea. Strain CB4Twas found to be strictly aerobic, Gram-stain-positive, rod-shaped, motile and formed creamy greyish colonies on nutrient agar. The major fatty acids were identified as iso-C15 : 0and iso-C16 : 0, and the predominant isoprenoid quinone as MK-7. The cell-wall peptidoglycan contained glycine and alanine as the diagnostic amino acids and phosphatidyl-Nmethylethanolamine, phosphatidylethanolamine, diphosphatidylglycerol and an unidentified aminophospholipid as the polar lipids. The genomic DNA G+C content of strain CB4Twas 46.5 mol%. Phylogenetic analysis, based on 16S rRNA gene sequence similarities, showed that strain CB4Tforms a deep branch within the genus Aneurinibacillus, sharing the highest level of sequence homology with Aneurinibacillus aneurinilyticus DSM 5562T(96.5 %). On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, strain CB4Tis considered to represent a novel species within the genus Aneurinibacillus, for which the name Aneurinibacillus soli sp. nov. is proposed. The type strain is CB4T(=KCTC 33505T=CECT 8566T). An emended description of the genus Aneurinibacillus is also proposed.