Effects of hydroxysafflor yellow A on ALDH1: Inhibition kinetics and molecular dynamics simulation

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Title
Effects of hydroxysafflor yellow A on ALDH1: Inhibition kinetics and molecular dynamics simulation
Author(s)
X Zhang; D Shen; Z R Lu; Y Zhan; N Si; M M Li; J M Yang; H M Zhou; Y D Park; Q Zhang; Jinhyuk Lee
Bibliographic Citation
Process Biochemistry, vol. 49, no. 10, pp. 1664-1672
Publication Year
2014
Abstract
Hydroxysafflor yellow A (HSYA) is a potent natural antioxidant that displays important neuroprotective activity. Inhibition of acetaldehyde dehydrogenase 1 (ALDH1) has attracted the attention of researchers due to its overexpression in several types of cancers. We studied the effects of HSYA on ALDH1 by evaluating the inhibitory kinetics based on its antioxidant properties and performing computational simulation integrating methods. HSYA reversibly inhibited human recombinant ALDH1 via non-competitive inhibition (Ki = 0.267 ± 0.024 mM). We also investigated the tertiary structural changes via measuring intrinsic and ANS-binding fluorescence. The results indicated that the inactivation induced by HSYA was associated with structural changes. To obtain further information, we simulated the 3D structure of ALDH1 and conducted computational docking simulations as well as molecular dynamics simulations. The results indicated that 4 rings of HSYA interact with several residues near the ALDH1 active site. Our study provides insight into the inhibition of ALDH1 accompanied by structural changes. Based on its ALDH1-inhibiting effect and its potential as a natural antioxidant, HSYA is a potential agent for treating ALDH1-associated cancers.
Keyword
ALDH1Docking simulationHydroxysafflor yellow AInhibition kineticsMolecular dynamicsNon-competitive inhibitor
ISSN
0032-9592
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.procbio.2014.06.010
Type
Article
Appears in Collections:
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
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