Effect of lithium chloride on the production and sialylation of Fc-fusion protein in Chinese hamster ovary cell culture = Lithium chloride가 당단백질의 생산성과 당화에 미치는 영향

Cited 28 time in scopus
Metadata Downloads
Title
Effect of lithium chloride on the production and sialylation of Fc-fusion protein in Chinese hamster ovary cell culture = Lithium chloride가 당단백질의 생산성과 당화에 미치는 영향
Author(s)
T K Ha; Yeon-Gu Kim; G M Lee
Bibliographic Citation
Applied Microbiology and Biotechnology, vol. 98, no. 22, pp. 9239-9248
Publication Year
2014
Abstract
Lithium chloride (LiCl), which is a specific inhibitor of glycogen synthase kinase-3β, is known to induce cell cycle arrest at the G2/M phase and to regulate apoptosis. To determine the potential of LiCl as a chemical additive to enhance specific productivity (qp) of recombinant Chinese hamster ovary (rCHO) cells through cell cycle arrest at G2/M phase, rCHO cells producing Fc-fusion protein were cultivated in serum-free media with LiCl concentrations ranging from 0 to 20 mM. The addition of LiCl induced cell cycle arrest at G2/M phase and thereby decreased the specific cell growth rate. However, LiCl increased qp in a dose-dependent manner. The beneficial effect of LiCl on qp outweighed its detrimental effect on μ, resulting in improved maximum Fc-fusion protein concentration (MFPC) at 10 mM LiCl. The qp and MFPC in the bioreactor culture with 10 mM LiCl were 5.0 and 2.1 times higher than those without LiCl, respectively. In addition, the presence of LiCl at 10 mM did not significantly affect either intracellular α2,3-ST or extracellular sialidase activity. LiCl also inhibited apoptosis of cells in the decline phase of growth by increasing Bcl-2 expression. Taken together, the results obtained in this study demonstrate the potential of LiCl as a qp-enhancing additive in CHO cell culture for improved recombinant protein production. ⓒ 2014, Springer-Verlag Berlin Heidelberg.
Keyword
Cell cycleChinese hamster ovary cellLithium chlorideSialylationSpecific productivity
ISSN
0175-7598
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s00253-014-6012-0
Type
Article
Appears in Collections:
Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.