Quantification of homoegonol in rat plasma using liquid chromatography-tandem mass spectrometry and its pharmacokinetics application = 랫트 플라스마에서의 호모에고놀 정량

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Title
Quantification of homoegonol in rat plasma using liquid chromatography-tandem mass spectrometry and its pharmacokinetics application = 랫트 플라스마에서의 호모에고놀 정량
Author(s)
H U Jeong; S S kwon; D G Hwang; E N Kim; K Lee; Kyung Seop AhnSei-Ryang Oh; H S Lee
Bibliographic Citation
Biomedical Chromatography, vol. 28, no. 12, pp. 1816-1821
Publication Year
2014
Abstract
Homoegonol is a biologically active neolignan isolated from Styrax species with cytotoxic, antimicrobial, anti-inflammatory and anti-asthma activities. For the quantification of homoegonol in rat plasma, a selective and sensitive liquid chromatography-tandem mass spectrometric method was developed and validated for the first time using protein precipitation with methanol as a sample clean-up procedure. The analytes were separated in an Atlantis dC18 column using a gradient elution of methanol and 0.1% formic acid, and mass-to-charge ratios were determined in selective reaction monitoring mode using tandem mass spectrometry with m/z 343.12>296.97 for homoegonol and m/z 517.30>282.90 for udenafil (internal standard). The standard curve was linear over the concentration ranges of 1-500ng/mL using a 30μL rat plasma sample. The coefficient of variation and relative error for intra- and inter-assay at four quality control levels were 3.9-10.0 and -3.3-2.7%, respectively. The overall recovery of homoegonol from rat plasma using protein precipitation was 99.7±7.7%. The pharmacokinetics parameters of homoegonol were dose-independent after both intravenous (1, 2.5 and 5mg/kg doses) and oral (5, 10 and 20mg/kg doses) administration in male Sprague-Dawley rats.
Keyword
HomoegonolLiquid chromatography-tandem mass spectrometryPharmacokineticsRat plasma
ISSN
0269-3879
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/bmc.3225
Type
Article
Appears in Collections:
Ochang Branch Institute > Natural Product Research Center > 1. Journal Articles
Ochang Branch Institute > 1. Journal Articles
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