Induction of clusterin expression by neuronal cell death in zebrafish

Cited 8 time in scopus
Metadata Downloads
Title
Induction of clusterin expression by neuronal cell death in zebrafish
Author(s)
Y M Jeong; Tae Eun Jin; J H Choi; M S Lee; H T Kim; G S Hwang; Doo-Sang ParkHyun Woo Oh; J K Choi; V Korzh; M Schachner; K H You; C H Kim
Bibliographic Citation
Journal of Genetics and Genomics, vol. 41, no. 11, pp. 583-589
Publication Year
2014
Abstract
Clusterin, a protein associated with multiple functions, is expressed in a wide variety of mammalian tissues. Although clusterin is known to be involved in neurodegenerative diseases, ageing, and tumorigenesis, a detailed analysis of the consequences of gain- or loss-of-function approaches has yet to be performed to understand the underlying mechanisms of clusterin functions. Since clusterin levels change in neurological diseases, it is likely that clusterin contributes to cell death and degeneration in general. Zebrafish was investigated as a model system to study human diseases. During development, zebrafish clusterin was expressed in the notochord and nervous system. Embryonic overexpression of clusterin by mRNA microinjection did not affect axis formation, whereas its knock-down by anti-sense morpholino treatment resulted in neuronal cell death. To analyze the function of clusterin in neurodegeneration, a transgenic zebrafish was investigated, in which nitroreductase expression is regulated under the control of a neuron-specific huC promoter which is active between the stages of early neuronal precursors and mature neurons. Nitroreductase turns metronidazole into a cytotoxic agent that induces cell death within 12h. After metronidazole treatment, transgenic zebrafish showed neuron-specific cell death. Interestingly, we also observed a dramatic induction of clusterin expression in the brain and spinal cord in these fish, suggesting a direct or indirect role of clusterin in neuronal cell death and thus, more generally, in neurodegeneration.
Keyword
ZebrafishClusterinNeurodegenerationNeuronal cell death
ISSN
1673-8527
Publisher
Science Press
DOI
http://dx.doi.org/10.1016/j.jgg.2014.08.007
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Division of Bio Technology Innovation > Core Research Facility & Analysis Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.