Enzymatic biosynthesis of novel resveratrol glucoside and glycoside derivatives

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Enzymatic biosynthesis of novel resveratrol glucoside and glycoside derivatives
R P Pandey; P Parajuli; J Y Shin; J Lee; S Lee; Young-Soo Hong; Y I Park; Joong Su Kim; J K Sohng
Bibliographic Citation
Applied and Environmental Microbiology, vol. 80, no. 23, pp. 7235-7243
Publication Year
A UDP glucosyltransferase from Bacillus licheniformis was overexpressed, purified, and incubated with nucleotide diphosphate (NDP) D- and L-sugars to produce glucose, galactose, 2-deoxyglucose, viosamine, rhamnose, and fucose sugar-conjugated resveratrol glycosides. Significantly higher (90%) bioconversion of resveratrol was achieved with α-D-glucose as the sugar donor to produce four different glucosides of resveratrol: resveratrol 3-O-β-D-glucoside, resveratrol 4'-O-β-D-glucoside, resveratrol 3,5- O-β-D-diglucoside, and resveratrol 3,5,4'-O-β-D-triglucoside. The conversion rates and numbers of products formed were found to vary with the other NDP sugar donors. Resveratrol 3-O-β-D-2-deoxyglucoside and resveratrol 3,5-O-β-D-di-2-deoxyglucoside were found to be produced using TDP-2-deoxyglucose as a donor; however, the monoglycosides resveratrol 4'-O-β-D-galactoside, resveratrol 4'-O-β-D-viosaminoside, resveratrol 3-O-β-L-rhamnoside, and resveratrol 3-O-β-L-fucoside were produced from the respective sugar donors. Altogether, 10 diverse glycoside derivatives of the medically important resveratrol were generated, demonstrating the capacity of YjiC to produce structurally diverse resveratrol glycosides.
Amer Soc Microb
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Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
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