Analysis of S-locus and expression of S-alleles of self-compatible rapid-cycling Brassica oleracea 'TO1000DH3'

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dc.contributor.authorH J Jung-
dc.contributor.authorN U Ahmed-
dc.contributor.authorJ I Park-
dc.contributor.authorS K Thamilarasan-
dc.contributor.authorHyeran Kim-
dc.contributor.authorY G Cho-
dc.contributor.authorI S Nou-
dc.date.accessioned2017-04-19T09:59:45Z-
dc.date.available2017-04-19T09:59:45Z-
dc.date.issued2014-
dc.identifier.issn03014851-
dc.identifier.uri10.1007/s11033-014-3526-6ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12324-
dc.description.abstractBrassica oleracea is a strictly self-incompatible (SI) plant, but rapid-cycling B. oleracea ‘TO1000DH3’ is self-compatible (SC). Self-incompatibility in Brassicaceae is controlled by multiple alleles of the S-locus. Three S-locus genes, S-locus glycoprotein (SLG), S-locus receptor kinase (SRK) and S-locus protein 11 or S-locus cysteine-rich (SP11/SCR), have been reported to date, all of which are classified into class I and II. In this study, we investigated the molecular mechanism behind alterations of SI to SC in rapid-cycling B. olerace ‘TO1000DH3’. Class I SRK were identified by genomic DNA PCR and PCR-RFLP analysis using SRK specific markers and found to be homozygous. Cloning and sequencing of class I SRK revealed a normal kinase domain without any S-domain/transmembrane domain. Moreover, S-locus sequencing analysis revealed only an SLG sequence, but no SP11/SCR. Expression analysis showed no SRK expression in the stigma, although other genes involved in the SI recognition reaction (SLG,MLPK, ARC1, THL) were found to have normal expression in the stigma. Taken together, the above results suggest that structural aberrations such as deletion of the SI recognition genes may be responsible for the breakdown of SI in rapid-cycling B. oleracea ‘TO1000DH3’.-
dc.publisherSpringer-
dc.titleAnalysis of S-locus and expression of S-alleles of self-compatible rapid-cycling Brassica oleracea 'TO1000DH3'-
dc.title.alternativeAnalysis of S-locus and expression of S-alleles of self-compatible rapid-cycling Brassica oleracea 'TO1000DH3'-
dc.typeArticle-
dc.citation.titleMolecular Biology Reports-
dc.citation.number10-
dc.citation.endPage6448-
dc.citation.startPage6441-
dc.citation.volume41-
dc.contributor.affiliatedAuthorHyeran Kim-
dc.contributor.alternativeName정희정-
dc.contributor.alternativeNameAhmed-
dc.contributor.alternativeName박종인-
dc.contributor.alternativeNameThamilarasan-
dc.contributor.alternativeName김혜란-
dc.contributor.alternativeName조용구-
dc.contributor.alternativeName노일섭-
dc.identifier.bibliographicCitationMolecular Biology Reports, vol. 41, no. 10, pp. 6441-6448-
dc.identifier.doi10.1007/s11033-014-3526-6-
dc.subject.keywordRapid-cycling Brassica oleracea-
dc.subject.keywordS-locus genes-
dc.subject.keywordSelf-compatibility-
dc.subject.keywordStructural aberration-
dc.subject.localRapid-cycling Brassica oleracea-
dc.subject.localS-locus genes-
dc.subject.localSelf-compatibility-
dc.subject.localStructural aberration-
dc.description.journalClassY-
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Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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