Reptin regulates pluripotency of embryonic stem cells and somatic cell reprogramming through Oct-4-dependent mechanism

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dc.contributor.authorE K Do-
dc.contributor.authorH C Cheon-
dc.contributor.authorI H Jang-
dc.contributor.authorE J Choi-
dc.contributor.authorS C Heo-
dc.contributor.authorK T Kang-
dc.contributor.authorKwang-Hee Bae-
dc.contributor.authorYee Sook Cho-
dc.contributor.authorJ K Seo-
dc.contributor.authorJ H Yoon-
dc.contributor.authorT G Lee-
dc.contributor.authorJ H Kim-
dc.date.accessioned2017-04-19T09:59:45Z-
dc.date.available2017-04-19T09:59:45Z-
dc.date.issued2014-
dc.identifier.issn1066-5099-
dc.identifier.uri10.1002/stem.1827ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12326-
dc.description.abstractOct4 has been implicated in regulation of pluripotency in embryonic stem cells (ESCs) and reprogramming of somatic cells into induced pluripotent stem cells. However, the molecular mechanisms involved in Oct4-dependent regulation of pluripotency and reprogramming have not been clear. To gain insight into the mechanism of regulation of Oct4-mediated self-renewal of ESCs and reprogramming of somatic cells, we attempted to identify Oct4-binding proteins using affinity purification and mass spectrometry. We identified Reptin, a key component of ATP-dependent chromatin remodeling complexes, as an Oct4-binding protein. Depletion of endogenous Reptin using lentiviral short hairpin RNA (shRNA) led to a decrease in the number and size of alkaline phosphatase-positive colonies of mouse ESCs. In addition, shRNA-mediated silencing of Reptin resulted in decreased expression of pluripotency-specific marker genes, including Oct4, Sox2, Nanog, and SSEA-1. Results of the Oct4 reporter assay showed synergism between Oct4 and Reptin, and depletion of endogenous Reptin abolished Oct4 transcriptional activity. Results of a chromatin immunoprecipitation assay showed the overlapping interaction of Reptin and Oct4 to CR4 in the Oct4 enhancer in ESCs. Knockdown of Reptin using shRNA suppressed the reprogramming of mouse embryonic fibroblasts to induced pluripotent stem cells, whereas overexpression of Reptin resulted in enhanced efficiency of induced pluripotent stem cell generation. These results strongly suggest that Reptin plays a key role in maintaining the pluripotency of ESCs and in establishing the pluripotency during reprogramming of somatic cells by regulation of Oct4-mediated gene regulation. Stem Cells 2014;32:3126-3136-
dc.publisherWiley-
dc.titleReptin regulates pluripotency of embryonic stem cells and somatic cell reprogramming through Oct-4-dependent mechanism-
dc.title.alternativeReptin regulates pluripotency of embryonic stem cells and somatic cell reprogramming through Oct-4-dependent mechanism-
dc.typeArticle-
dc.citation.titleStem Cells-
dc.citation.number12-
dc.citation.endPage3136-
dc.citation.startPage3126-
dc.citation.volume32-
dc.contributor.affiliatedAuthorKwang-Hee Bae-
dc.contributor.affiliatedAuthorYee Sook Cho-
dc.contributor.alternativeName도은경-
dc.contributor.alternativeName전효천-
dc.contributor.alternativeName장일호-
dc.contributor.alternativeName최은정-
dc.contributor.alternativeName허순철-
dc.contributor.alternativeName강경택-
dc.contributor.alternativeName배광희-
dc.contributor.alternativeName조이숙-
dc.contributor.alternativeName서정곤-
dc.contributor.alternativeName윤종혁-
dc.contributor.alternativeName이태훈-
dc.contributor.alternativeName김재호-
dc.identifier.bibliographicCitationStem Cells, vol. 32, no. 12, pp. 3126-3136-
dc.identifier.doi10.1002/stem.1827-
dc.subject.keywordEmbryonic stem cells-
dc.subject.keywordInduced pluripotent stem-
dc.subject.keywordOct4-
dc.subject.keywordReprogramming-
dc.subject.keywordReptin-
dc.subject.localEmbryonic stem cell-
dc.subject.localEmbryonic stem cells-
dc.subject.localembryonic stem cell-
dc.subject.localembryonic stem cell (ESC)-
dc.subject.localembryonic stem cells-
dc.subject.localInduced pluripotent stem-
dc.subject.localOct-4-
dc.subject.localOct4-
dc.subject.localReprogramming-
dc.subject.localreprogramming-
dc.subject.localReptin-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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