Characterization of glutamate decarboxylase from Lactobacillus plantarum and its C-terminal function for the pH dependence of activity

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dc.contributor.authorS M Shin-
dc.contributor.authorH Kim-
dc.contributor.authorY Joo-
dc.contributor.authorS J Lee-
dc.contributor.authorY J Lee-
dc.contributor.authorSang Jun Lee-
dc.contributor.authorD W Lee-
dc.date.accessioned2017-04-19T10:00:02Z-
dc.date.available2017-04-19T10:00:02Z-
dc.date.issued2014-
dc.identifier.issn0021-8561-
dc.identifier.uri10.1021/jf504656hko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12335-
dc.description.abstractThe gadB gene encoding glutamate decarboxylase (GAD) from Lactobacillus plantarum was cloned and expressed in Escherichia coli. The recombinant enzyme exhibited maximal activity at 40 °C and pH 5.0. The 3D model structure of L. plantarum GAD proposed that its C-terminal region (Ile454-Thr468) may play an important role in the pH dependence of catalysis. Accordingly, C-terminally truncated (Δ3 and Δ11 residues) mutants were generated and their enzyme activities compared with that of the wild-type enzyme at different pH values. Unlike the wild-type GAD, the mutants showed pronounced catalytic activity in a broad pH range of 4.0-8.0, suggesting that the C-terminal region is involved in the pH dependence of GAD activity. Therefore, this study may provide effective target regions for engineering pH dependence of GAD activity, thereby meeting industrial demands for the production of γ-aminobutyrate in a broad range of pH values.-
dc.publisherAmer Chem Soc-
dc.titleCharacterization of glutamate decarboxylase from Lactobacillus plantarum and its C-terminal function for the pH dependence of activity-
dc.title.alternativeCharacterization of glutamate decarboxylase from Lactobacillus plantarum and its C-terminal function for the pH dependence of activity-
dc.typeArticle-
dc.citation.titleJournal of Agricultural and Food Chemistry-
dc.citation.number50-
dc.citation.endPage12193-
dc.citation.startPage12186-
dc.citation.volume62-
dc.contributor.affiliatedAuthorSang Jun Lee-
dc.contributor.alternativeName신선미-
dc.contributor.alternativeName김하나-
dc.contributor.alternativeName주윤혜-
dc.contributor.alternativeName이상재-
dc.contributor.alternativeName이용직-
dc.contributor.alternativeName이상준-
dc.contributor.alternativeName이동우-
dc.identifier.bibliographicCitationJournal of Agricultural and Food Chemistry, vol. 62, no. 50, pp. 12186-12193-
dc.identifier.doi10.1021/jf504656h-
dc.subject.keywordC-terminal region-
dc.subject.keywordGABA-
dc.subject.keywordGlutamate decarboxylase-
dc.subject.keywordLactobacillus plantarum-
dc.subject.keywordPH dependence-
dc.subject.localC-terminal region-
dc.subject.localGABA-
dc.subject.localGlutamate decarboxylase-
dc.subject.localLactobacillus plantarum-
dc.subject.localPH dependence-
dc.description.journalClassY-
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