Litoreibacter ponti sp. nov., Isolated from seawater

Abstract

A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated GJSW-31T, was isolated from seawater from the South Sea, South Korea. The nov.l strain grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0% (w/v) NaCl. Phylogenetic trees, based on 16S rRNA gene sequences, revealed that strain GJSW-31T clustered with the type strains of sp.cies of the genus Litoreibacter. Strain GJSW-31T exhibited 16S rRNA gene sequence similarity values of 95.2-98.5% to the type strains of sp.cies of the genus Litoreibacter and sequence similarities of less than 96.18% to type strains of the other sp.cies with validly published names. Strain GJSW-31Tcontained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. The major polar lipids of strain GJSW-31T were phosphatidylcholine, phosphatidylglycerol, one unidentified lipid and one unidentified aminolipid. The DNA G+C content of strain GJSW-31Twas 62.5 mol% and its DNA-DNA relatedness values with the type strains of Litoreibacter albidus, Litoreibacter janthinus, Litoreibacter meonggei and Litoreibacter ascidiaceicola were 13-23 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain GJSW-31Tis separate from other sp.cies of the genus Litoreibacter. On the basis of the data presented, strain GJSW-31Tis considered to represent a nov.l sp.cies of the genus Litoreibacter, for which the name Litoreibacter ponti sp. nov. is proposed. The type strain is GJSW-31T(=KCTC 42114T=NBRC 110379T).