Arthrobacter enclensis sp. nov., isolated from sediment sample

Abstract

A nov.l bacterial strain designated as NIO-1008T was isolated from marine sediments sample in Chorao Island India. Cells of the strains were gram positive and non-motile, displayed a rod-coccus life cycle and formed cream to light grey colonies on nutrient agar. Strain NIO-1008T had the chemotaxonomic markers that were consistent for classification in the genus Arthrobacter, i.e. MK-9(H2) (50.3 %), as the major menaquinone, and the minor amount of MK-7 (H2-27.5 %), MK-8 (H4-11.6 %) and MK-8 (H2-10.4 %). anteiso-C15:0, iso-C15:0, iso-C16:0 and C15:0 were the predominant fatty acids. Galactose, glucose and rhamnose are the cell-wall sugars, and DNA G+C content was 61.3 mol%. Phylogenetic analysis, based on 16S rRNA gene sequencing, showed that the strains were most similar to Arthrobacter equi IMMIB L-1606T, Arthrobacter chlorophenolicus DSM 12829T, Arthrobacter defluvii KCTC 19209T and Arthrobacter niigatensis CCTCC AB 206012T with 98.5, 98.4, 98.0 and 97.8 %, respectively, and formed a separate lineage. Combined phenotypic data and DNA-DNA hybridization data supported the conclusion that strains NIO-1008T represent a nov.l sp.cies within the genus Arthrobacter, for which the name Arthrobacter enclensis sp. nov., is proposed. The type strain is NIO-1008T = (NCIM 5488T = DSM 25279T).