Improvement of fermentative production of exopolysaccharides from Aureobasidium pullulans under various conditions

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Title
Improvement of fermentative production of exopolysaccharides from Aureobasidium pullulans under various conditions
Author(s)
C Seo; Hong-Weon Lee; A Suresh; J W Yang; Joon Ki Jung; Y C Kim
Bibliographic Citation
Korean Journal of Chemical Engineering, vol. 31, no. 8, pp. 1433-1437
Publication Year
2014
Abstract
The optimization of exopolysaccharide (EPS) production was investigated in the polymorphic fungal strain of Aureobasidium pullulans (KCTC 6081) with varying pH, nutrients concentration, and mixing parameters in batch fermentation condition. The maximum production of EPS (∼7.5 g/L) was observed at pH 4, while optimum nutrient concentration of carbon (sucrose), nitrogen (NaNO3), phosphorous (K2HPO4), and ascorbic acid was 50 g/L, 5 g/L, 1 g/L and 2 g/L, respectively. Interestingly, EPS productivity under non pH controlled fermentation conditions was 0.12 g/L/h with 400 rpm mixing, while under a controlled pH of 4, the EPS productivity was 0.21 g/L/h with 600 rpm, respectively. The fed-batch fermentation increased the EPS productivity up to 0.345 g/L/h with changing mixing conditions from 200 to 600 rpm and reached 47 g/L with 88% pullulan. Thus, pH and mixing were the key parameters for enhancing EPS production from A. pullulans. It is expected that these optimized parameters can be well used for enhanced industrial production of pullulan.
Keyword
AgitationExopolysaccharideFermentationNutrientsOptimization
ISSN
I000-0127
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.1007/s11814-014-0064-9
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > 1. Journal Articles
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