Inhibitory effects of Hwang-Ryun-Hae-Dok-Tang on cytochrome P450 in human liver microsomes

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dc.contributor.authorS Y Lee-
dc.contributor.authorH Jang-
dc.contributor.authorJ Y Lee-
dc.contributor.authorJ Y Ma-
dc.contributor.authorSoo Jin Oh-
dc.contributor.authorS K Kim-
dc.date.accessioned2017-04-19T10:00:47Z-
dc.date.available2017-04-19T10:00:47Z-
dc.date.issued2015-
dc.identifier.issn0049-8254-
dc.identifier.uri10.3109/00498254.2014.953624ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12402-
dc.description.abstract1.The herb-drug interaction potential of Hwang-Ryun-Hae-Dok-Tang (HR) extracts mediated by cytochrome P450 (CYP) inhibition was determined using human liver microsomes. 2.HR strongly inhibited CYP1A2 and moderately inhibited CYP2C19, CYP2D6, and CYP3A4 (testosterone) but not CYP2A6, CYP2B6, CYP2C8, CYP2C9, and CYP3A4 (midazolam). 3.The enzyme kinetic results suggest that CYP1A2 inhibition is competitively reversible (Ki, 13.4±1.8μg/ml), and CYP2D6 inhibition is quasi-irreversible (KI, 0.234±0.138μg/ml; kinact, 0.067±0.006min-1). 4.Fermentation using Lactobacillus acidophilus attenuated the HR-induced inhibition of CYP2D6, but not the other isoforms. 5.Neither CYP1A2 nor CYP3A4 was markedly inhibited by berberine, palmatine, and geniposide major components in HR and CYP2D6 was inhibited by berberine (IC50, 13.8μg/ml) in a metabolism-dependent manner. 6.The results suggest the possibility of HR-drug interaction through inhibition of CYP particularly CYP2D6 which may be attenuated by fermentation using L. acidophilus.-
dc.publisherT&F (Taylor & Francis)-
dc.titleInhibitory effects of Hwang-Ryun-Hae-Dok-Tang on cytochrome P450 in human liver microsomes-
dc.title.alternativeInhibitory effects of Hwang-Ryun-Hae-Dok-Tang on cytochrome P450 in human liver microsomes-
dc.typeArticle-
dc.citation.titleXenobiotica-
dc.citation.number2-
dc.citation.endPage138-
dc.citation.startPage131-
dc.citation.volume45-
dc.contributor.affiliatedAuthorSoo Jin Oh-
dc.contributor.alternativeName이상윤-
dc.contributor.alternativeName장힘찬-
dc.contributor.alternativeName이지윤-
dc.contributor.alternativeName마진열-
dc.contributor.alternativeName오수진-
dc.contributor.alternativeName김상겸-
dc.identifier.bibliographicCitationXenobiotica, vol. 45, no. 2, pp. 131-138-
dc.identifier.doi10.3109/00498254.2014.953624-
dc.subject.keywordCytochrome P450-
dc.subject.keywordFermentation-
dc.subject.keywordHerb-drug interaction-
dc.subject.keywordHwang-Ryun-Hae-Dok-Tang-
dc.subject.localCytochrome P450s-
dc.subject.localCytochrome p450-
dc.subject.localCytochrome P450-
dc.subject.localcytochrome P450s-
dc.subject.localcytochrome P-450-
dc.subject.localcytochrome P450-
dc.subject.localfermentation-
dc.subject.localFermentation-
dc.subject.localHerb-drug interaction-
dc.subject.localHwangRyunHaeDok-tang-
dc.subject.localHwang-Ryun-Hae-Dok-Tang-
dc.description.journalClassY-
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