DC Field | Value | Language |
---|---|---|
dc.contributor.author | J H Kim | - |
dc.contributor.author | P Gripon | - |
dc.contributor.author | F Bouezzedine | - |
dc.contributor.author | M S Jeong | - |
dc.contributor.author | Seung-Wook Chi | - |
dc.contributor.author | S E Ryu | - |
dc.contributor.author | H J Hong | - |
dc.date.accessioned | 2017-04-19T10:01:04Z | - |
dc.date.available | 2017-04-19T10:01:04Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 0014-5793 | - |
dc.identifier.uri | 10.1016/j.febslet.2014.11.046 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/12418 | - |
dc.description.abstract | To improve a previously constructed broadly neutralizing hepatitis B virus (HBV)-specific preS1 humanized antibody (HzKR127), we further humanized it through specificity-determining residue (SDR) grafting. Moreover, we improved affinity by mutating two residues in heavy-chain complementarity-determining regions (CDR), on the basis of the crystal structure of the antigen-antibody complex. HzKR127-3.2 exhibited 2.5-fold higher affinity and enhanced virus-neutralizing activity compared to the original KR127 antibody and showed less immunogenic potential than HzKR127. Enhanced virus-neutralizing activity was achieved by the increased association rate, providing insights into engineering potent antibody therapeutics for HBV immunoprophylaxis. HzKR127-3.2 may be a good candidate for HBV immunoprophylaxis. | - |
dc.publisher | Wiley | - |
dc.title | Enhanced humanization and affinity maturation of neutralizing anti-hepatitis B virus preS1 antibody based on antigen-antibody complex structure | - |
dc.title.alternative | Enhanced humanization and affinity maturation of neutralizing anti-hepatitis B virus preS1 antibody based on antigen-antibody complex structure | - |
dc.type | Article | - |
dc.citation.title | FEBS Letters | - |
dc.citation.number | 2 | - |
dc.citation.endPage | 200 | - |
dc.citation.startPage | 193 | - |
dc.citation.volume | 589 | - |
dc.contributor.affiliatedAuthor | Seung-Wook Chi | - |
dc.contributor.alternativeName | 김진홍 | - |
dc.contributor.alternativeName | Gripon | - |
dc.contributor.alternativeName | Bouezzedine | - |
dc.contributor.alternativeName | 정문식 | - |
dc.contributor.alternativeName | 지승욱 | - |
dc.contributor.alternativeName | 류성언 | - |
dc.contributor.alternativeName | 홍효정 | - |
dc.identifier.bibliographicCitation | FEBS Letters, vol. 589, no. 2, pp. 193-200 | - |
dc.identifier.doi | 10.1016/j.febslet.2014.11.046 | - |
dc.subject.keyword | Affinity maturation | - |
dc.subject.keyword | Hepatitis B virus | - |
dc.subject.keyword | Humanized antibody | - |
dc.subject.keyword | preS1 | - |
dc.subject.keyword | Specificity-determining residue grafting | - |
dc.subject.keyword | Virus neutralization | - |
dc.subject.local | affinity maturation | - |
dc.subject.local | affinity Maturation | - |
dc.subject.local | Affinity maturation | - |
dc.subject.local | hepatitis B virus (HBV) | - |
dc.subject.local | Hepatitis B Virus | - |
dc.subject.local | Hepatitis B virus (HBV) | - |
dc.subject.local | hepatitis B virus | - |
dc.subject.local | hepatitis B Virus (HBV) | - |
dc.subject.local | Hepatitis B virus | - |
dc.subject.local | Humanized antibody | - |
dc.subject.local | humanized antibodies | - |
dc.subject.local | humanized antibody | - |
dc.subject.local | PreS1 | - |
dc.subject.local | preS1 | - |
dc.subject.local | Specificity-determining residue grafting | - |
dc.subject.local | Virus neutralization | - |
dc.description.journalClass | Y | - |
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