Quantitative expression analysis of APP pathway and tau phosphorylation-related genes in the ICV STZ-induced non-human primate model of sporadic Alzheimer’s disease

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dc.contributor.authorSang Je Park-
dc.contributor.authorYoung-Hyun Kim-
dc.contributor.authorGyu-Hwi Nam-
dc.contributor.authorSe Hee Choe-
dc.contributor.authorSang-Rae Lee-
dc.contributor.authorSun-Uk Kim-
dc.contributor.authorJi-Su Kim-
dc.contributor.authorBo Woong Sim-
dc.contributor.authorBong Seok Song-
dc.contributor.authorKang Jin Jeong-
dc.contributor.authorYoungjeon Lee-
dc.contributor.authorY I Park-
dc.contributor.authorK M Lee-
dc.contributor.authorJae Won Huh-
dc.contributor.authorKyu Tae Chang-
dc.date.accessioned2017-04-19T10:01:28Z-
dc.date.available2017-04-19T10:01:28Z-
dc.date.issued2015-
dc.identifier.issn14220067-
dc.identifier.uri10.3390/ijms16022386ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12426-
dc.description.abstractThe accumulation and aggregation of misfolded proteins in the brain, such as amyloid-β (Aβ) and hyperphosphorylated tau, is a neuropathological hallmark of Alzheimer’s disease (AD). Previously, we developed and validated a novel non-human primate model for sporadic AD (sAD) research using intracerebroventricular administration of streptozotocin (icv STZ). To date, no characterization of AD-related genes in different brain regions has been performed. Therefore, in the current study, the expression of seven amyloid precursor protein (APP) pathway-related and five tau phosphorylation-related genes was investigated by quantitative real-time PCR experiments, using two matched-pair brain samples from control and icv STZ-treated cynomolgus monkeys. The genes showed similar expression patterns within the control and icv STZ-treated groups; however, marked differences in gene expression patterns were observed between the control and icv STZ-treated groups. Remarkably, other than β-secretase (BACE1) and cyclin-dependent kinase 5 (CDK5), all the genes tested showed similar expression patterns in AD models compared to controls, with increased levels in the precuneus and occipital cortex. However, significant changes in gene expression patterns were not detected in the frontal cortex, hippocampus, or posterior cingulate. Based on these results, we conclude that APP may be cleaved via the general metabolic mechanisms of increased α- and γ-secretase levels, and that hyperphosphorylation of tau could be mediated by elevated levels of tau protein kinase, specifically in the precuneus and occipital cortex.-
dc.publisherMDPI-
dc.titleQuantitative expression analysis of APP pathway and tau phosphorylation-related genes in the ICV STZ-induced non-human primate model of sporadic Alzheimer’s disease-
dc.title.alternativeQuantitative expression analysis of APP pathway and tau phosphorylation-related genes in the ICV STZ-induced non-human primate model of sporadic Alzheimer’s disease-
dc.typeArticle-
dc.citation.titleInternational Journal of Molecular Sciences-
dc.citation.number2-
dc.citation.endPage2402-
dc.citation.startPage2386-
dc.citation.volume16-
dc.contributor.affiliatedAuthorSang Je Park-
dc.contributor.affiliatedAuthorYoung-Hyun Kim-
dc.contributor.affiliatedAuthorSang-Rae Lee-
dc.contributor.affiliatedAuthorSun-Uk Kim-
dc.contributor.affiliatedAuthorJi-Su Kim-
dc.contributor.affiliatedAuthorBo Woong Sim-
dc.contributor.affiliatedAuthorBong Seok Song-
dc.contributor.affiliatedAuthorKang Jin Jeong-
dc.contributor.affiliatedAuthorYoungjeon Lee-
dc.contributor.affiliatedAuthorJae Won Huh-
dc.contributor.alternativeName박상제-
dc.contributor.alternativeName김영현-
dc.contributor.alternativeName남규휘-
dc.contributor.alternativeName최세희-
dc.contributor.alternativeName이상래-
dc.contributor.alternativeName김선욱-
dc.contributor.alternativeName김지수-
dc.contributor.alternativeName심보웅-
dc.contributor.alternativeName송봉석-
dc.contributor.alternativeName정강진-
dc.contributor.alternativeName이영전-
dc.contributor.alternativeName박영일-
dc.contributor.alternativeName이경민-
dc.contributor.alternativeName허재원-
dc.contributor.alternativeName장규태-
dc.identifier.bibliographicCitationInternational Journal of Molecular Sciences, vol. 16, no. 2, pp. 2386-2402-
dc.identifier.doi10.3390/ijms16022386-
dc.subject.keywordAlzheimer’s disease-
dc.subject.keywordAPP-
dc.subject.keywordCynomolgus monkey-
dc.subject.keywordqPCR-
dc.subject.keywordStreptosozocin-
dc.subject.keywordTau-
dc.subject.localAlzheimer’s disease-
dc.subject.localAPP-
dc.subject.localCynomolgus monkey-
dc.subject.localqPCR-
dc.subject.localStreptosozocin-
dc.subject.localTau-
dc.description.journalClassY-
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > National Primate Research Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Primate Resources Center > 1. Journal Articles
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