The CpxRA two-component system is involved in the maintenance of the integrity of the cell envelope in the rumen bacterium Mannheimia succiniciproducens

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dc.contributor.authorSeulgi Yun-
dc.contributor.authorEun Gyeong Lee-
dc.contributor.authorSang Yoon Kim-
dc.contributor.authorJong Moon Shin-
dc.contributor.authorWon Seok Jung-
dc.contributor.authorDoo-Byoung Oh-
dc.contributor.authorS Y Lee-
dc.contributor.authorOh Suk Kwon-
dc.date.accessioned2017-04-19T10:01:42Z-
dc.date.available2017-04-19T10:01:42Z-
dc.date.issued2015-
dc.identifier.issn03438651-
dc.identifier.uri10.1007/s00284-014-0686-5ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12439-
dc.description.abstractIn this study, we characterized the CpxRA two-component signal transduction system of the rumen bacterium Mannheimia succiniciproducens. The truncated form of the CpxA sensor kinase protein without its transmembrane domain was able to autophosphorylate and transphosphorylate the CpxR response regulator protein in vitro. We identified 152 putative target genes for the Cpx system in M. succiniciproducens, which were differentially expressed by more than twofold upon overexpression of the CpxR protein. Genes of a putative 16-gene operon related to the cell wall and lipopolysaccharide biosynthesis were induced strongly upon CpxR overexpression. The promoter region of the first gene of this operon, wecC encoding UDP-N-acetyl-D-mannosaminuronate dehydrogenase, was analyzed and found to contain a sequence homologous to the CpxR box of Escherichia coli. An electrophoretic mobility shift assay showed that the phosphorylated CpxR proteins were able to bind specifically to PCR-amplified DNA fragments containing the promoter sequence of wecC. Furthermore, a cpxR-disrupted mutant strain exhibited increased envelope permeability compared with a wild-type strain. These results suggest that the Cpx system of M. succiniciproducens is involved in the maintenance of the integrity of the cell envelope.-
dc.publisherSpringer-
dc.titleThe CpxRA two-component system is involved in the maintenance of the integrity of the cell envelope in the rumen bacterium Mannheimia succiniciproducens-
dc.title.alternativeThe CpxRA two-component system is involved in the maintenance of the integrity of the cell envelope in the rumen bacterium Mannheimia succiniciproducens-
dc.typeArticle-
dc.citation.titleCurrent Microbiology-
dc.citation.number1-
dc.citation.endPage109-
dc.citation.startPage103-
dc.citation.volume70-
dc.contributor.affiliatedAuthorDoo-Byoung Oh-
dc.contributor.affiliatedAuthorOh Suk Kwon-
dc.contributor.alternativeName윤슬기-
dc.contributor.alternativeName이은경-
dc.contributor.alternativeName김상윤-
dc.contributor.alternativeName신종문-
dc.contributor.alternativeName정원석-
dc.contributor.alternativeName오두병-
dc.contributor.alternativeName이상엽-
dc.contributor.alternativeName권오석-
dc.identifier.bibliographicCitationCurrent Microbiology, vol. 70, no. 1, pp. 103-109-
dc.identifier.doi10.1007/s00284-014-0686-5-
dc.description.journalClassY-
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Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
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