Gene expression profiling by RNA sequencing in mature/immature cocytes of chicken = 닭의 성숙/미성숙란에서 RNA sequencing을 이용한 유전자 발현 양상 고찰

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dc.contributor.authorK S Kang-
dc.contributor.authorH J Jang-
dc.contributor.authorM N Park-
dc.contributor.authorJ W Choi-
dc.contributor.authorWon-Hyong Chung-
dc.contributor.authorK N Heo-
dc.contributor.authorC Y Choe-
dc.contributor.authorY J Kim-
dc.contributor.authorS W Lee-
dc.contributor.authorE S Cho-
dc.contributor.authorNamshin Kim-
dc.contributor.authorT H Kim-
dc.contributor.authorJ Y Han-
dc.contributor.authorK T Lee-
dc.date.accessioned2017-04-19T10:01:42Z-
dc.date.available2017-04-19T10:01:42Z-
dc.date.issued2014-
dc.identifier.issn1225-6625-
dc.identifier.uri10.5536/KJPS.2014.41.4.287ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12441-
dc.description.abstractChicken eggs undergo various physiological changes during egg maturation. To study genes associated with the egg maturation in pre-ovulation (immature) and post-ovulation (mature), we compared gene expression patterns between in the immature egg and mature egg using RNA sequencing data. Mature and immature eggs were obtained from a Heuksaek Jaerae-jong of Korean native chicken. Total RNAs obtained from the eggs were sequenced by Illumina HiSeq 2000 platform, and the generated sequence reads were mapped to Galgal4 reference sequence assembly using Tuxedo Protocol. From the comparison of the RNA sequencing data, 315 genes were differentially expressed between mature and immature eggs, and 46 genes were only detected in immature egg. Further gene ontology (GO) analysis was performed for the differentially expressed genes using DAVID, showing that 29 and 28 GO terms were independently clustered from mature and immature, respectively. From those clustered GO terms, genes related to germ cell development, sex differentiation and defense response to bacterium were mainly expressed in the immature egg, while genes related to regulation of apoptosis, steroid metabolic process and lipid homeostasis were mainly detected in the mature egg. Our results could contribute to understand egg maturation before and after ovulation, and develop genetic markers for improving egg quality and productivity.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleGene expression profiling by RNA sequencing in mature/immature cocytes of chicken = 닭의 성숙/미성숙란에서 RNA sequencing을 이용한 유전자 발현 양상 고찰-
dc.title.alternativeGene expression profiling by RNA sequencing in mature/immature cocytes of chicken-
dc.typeArticle-
dc.citation.titleKorean Journal of Poultry Science-
dc.citation.number4-
dc.citation.endPage296-
dc.citation.startPage287-
dc.citation.volume41-
dc.contributor.affiliatedAuthorWon-Hyong Chung-
dc.contributor.affiliatedAuthorNamshin Kim-
dc.contributor.alternativeName강경수-
dc.contributor.alternativeName장현준-
dc.contributor.alternativeName박미나-
dc.contributor.alternativeName최정우-
dc.contributor.alternativeName정원형-
dc.contributor.alternativeName허강녕-
dc.contributor.alternativeName최창용-
dc.contributor.alternativeName김영주-
dc.contributor.alternativeName이시우-
dc.contributor.alternativeName조은석-
dc.contributor.alternativeName김남신-
dc.contributor.alternativeName김태헌-
dc.contributor.alternativeName한재용-
dc.contributor.alternativeName이경태-
dc.identifier.bibliographicCitationKorean Journal of Poultry Science, vol. 41, no. 4, pp. 287-296-
dc.identifier.doi10.5536/KJPS.2014.41.4.287-
dc.subject.keywordegg maturation-
dc.subject.keywordRNA sequencing-
dc.subject.keywordgene ontology-
dc.subject.keyworddifferential gene expression-
dc.subject.localegg maturation-
dc.subject.localRNA sequencing-
dc.subject.localRNA sequencing (RNA-seq)-
dc.subject.localrna sequence-
dc.subject.localGene ontology-
dc.subject.localgene ontology-
dc.subject.localGene Ontology-
dc.subject.localdifferential gene expression-
dc.description.journalClassN-
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