Enhanced production of 2,3-butanediol by a genetically engineered Bacillus sp. BRC1 using a hydrolysate of empty palm fruit bunches

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Title
Enhanced production of 2,3-butanediol by a genetically engineered Bacillus sp. BRC1 using a hydrolysate of empty palm fruit bunches
Author(s)
In Yeong Kang; Jang Min Park; Won-Kyung Hong; Yun Seok Kim; You Ree Jung; Seung Bum Kim; Sun-Yeon Heo; Sung Mok Lee; Ji Young KangBaek Rock Oh; Dae-Hyuk Kim; Jeong-Woo SeoChul Ho Kim
Bibliographic Citation
Bioprocess and Biosystems Engineering, vol. 38, no. 2, pp. 299-305
Publication Year
2015
Abstract
A Bacillus sp.cies that produces 2,3-butanediol (2,3-BD), termed BRC1, was newly isolated, and a 2,3-BD dehydrogenase (Bdh) from this sp.cies was identified and characterized at the molecular and biochemical level. Sequence analysis revealed that Bdh is homologous to D-2,3-BD dehydrogenases. An analysis of the enzymatic properties of Bdh overexpressed in Escherichia coli confirmed the molecular results, showing preferred activity toward D-2,3-BD. Optimum pH, temperature, and kinetics determined for reductive and oxidative reactions support the preferential production of 2,3-BD during cell growth. Overexpression of bdh under the control of a xylose-inducible promoter resulted in increased enzyme activity and enhanced 2,3-BD production in Bacillus sp. BRC1. Additionally, a hydrolysate of cellulosic material, (empty palm fruit bunches), was successfully used for the enhanced production of 2,3-BD in the recombinant Bacillus strain.
Keyword
2,3-butanediol2,3-butanediol dehydrogenaseBacillus sp.Empty palm fruit bunches
ISSN
1615-7591
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s00449-014-1268-4
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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