Long-term survival and differentiation of human neural stem cells in nonhuman primate brain with no immunosuppression

Cited 18 time in scopus
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Title
Long-term survival and differentiation of human neural stem cells in nonhuman primate brain with no immunosuppression
Author(s)
Sang-Rae Lee; H J Lee; S H Cha; Kang Jin JeongYoungjeon Lee; Chang Yeop Jeon; K S Yi; I Lim; Z H Cho; Kyu Tae Chang; S U Kim
Bibliographic Citation
Cell Transplantation, vol. 24, no. 2, pp. 191-201
Publication Year
2015
Abstract
Cellular fate of human neural stem cells (hNSCs) transplanted in the brain of nonhuman primates (NHPs) with no immunosuppression was determined at 22 and 24 months posttransplantation (PTx) regarding survival, differentiation, and tumorigenesis. Survival of hNSCs labeled with magnetic nanoparticles was successfully detected around injection sites in the brain at 22 months PTx by MRI. Histological examination of brain sections with H&E and Prussian blue staining at 24 months revealed that most of the grafted hNSCs were found located along the injection tract. Grafted hNSCs were found to differentiate into neurons at 24 months PTx. In addition, none of the grafted hNSCs were bromodeoxyuridine positive in the monkey brain, indicating that hNSCs did not replicate in the NHP brain and did not cause tumor formation. This study serves as a proof of principle and provides evidence that hNSCs transplanted in NHP brain could survive and differentiate into neurons in the absence of immunosuppression. It also serves as a preliminary study in our scheduled preclinical studies of hNSC transplantation in NHP stroke models.
Keyword
Brain transplantationHuman neural stem cells (hNSCs)Magnetic nanoparticle (MNP)Magnetic resonance imaging (MRI)Nonhuman primate (NHP)
ISSN
0963-6897
Publisher
Sage
DOI
http://dx.doi.org/10.3727/096368914X678526
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > National Primate Research Center > 1. Journal Articles
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