DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeong Hee Moon | - |
dc.contributor.author | S Yoon | - |
dc.contributor.author | Y J Bae | - |
dc.contributor.author | M S Kim | - |
dc.date.accessioned | 2017-04-19T10:02:01Z | - |
dc.date.available | 2017-04-19T10:02:01Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 0277-7037 | - |
dc.identifier.uri | 10.1002/mas.21427 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/12470 | - |
dc.description.abstract | Insights on mechanisms for the generation of gas-phase peptide ions and their dissociation in matrix-assisted laser desorption ionization (MALDI) gained from the kinetic and ion yield studies are presented. Even though the time-resolved photodissociation technique was initially used to determine the dissociation kinetics of peptide ions and their effective temperature, it was replaced by a simpler method utilizing dissociation yields from in-source decay (ISD) and post-source decay (PSD). The ion yields for a matrix and a peptide were measured by repeatedly irradiating a region on a sample and collecting ion signals until the sample in the region was completely depleted. Matrix- and peptide-derived gas-phase cations were found to be generated by pre-formed ion emission or by ion-pair emission followed by anion loss, but not by laser-induced ionization. The total number of ions, that is, matrix plus peptide, was found to be equal to the number of ions emitted from a pure matrix. A matrix plume was found to cool as it expanded, from around 800-1,000 K to 400-500 K. Dissociation of peptide ions along b/y channels was found to occur statistically, that is, following RRKM behavior. Small critical energy (E0=0.6-0.7 eV) and highly negative critical entropy (ΔS‡=-30 to -25 eu) suggested that the transition structure was stabilized by multiple intramolecular interactions. | - |
dc.publisher | Wiley | - |
dc.title | Formation of gas-phase peptide ions and their dissociation in MALDI: insights from kinetic and ion yield studies | - |
dc.title.alternative | Formation of gas-phase peptide ions and their dissociation in MALDI: insights from kinetic and ion yield studies | - |
dc.type | Article | - |
dc.citation.title | Mass Spectrometry Reviews | - |
dc.citation.number | 2 | - |
dc.citation.endPage | 115 | - |
dc.citation.startPage | 94 | - |
dc.citation.volume | 34 | - |
dc.contributor.affiliatedAuthor | Jeong Hee Moon | - |
dc.contributor.alternativeName | 문정희 | - |
dc.contributor.alternativeName | 윤소희 | - |
dc.contributor.alternativeName | 배용진 | - |
dc.contributor.alternativeName | 김명수 | - |
dc.identifier.bibliographicCitation | Mass Spectrometry Reviews, vol. 34, no. 2, pp. 94-115 | - |
dc.identifier.doi | 10.1002/mas.21427 | - |
dc.subject.keyword | Expansion cooling | - |
dc.subject.keyword | Ion yield | - |
dc.subject.keyword | MALDI | - |
dc.subject.keyword | Peptide ion dissociation | - |
dc.subject.keyword | Peptide ion formation | - |
dc.subject.keyword | Thermal model | - |
dc.subject.local | Expansion cooling | - |
dc.subject.local | Ion yield | - |
dc.subject.local | MALDI | - |
dc.subject.local | Peptide ion dissociation | - |
dc.subject.local | Peptide ion formation | - |
dc.subject.local | Thermal model | - |
dc.description.journalClass | Y | - |
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