Lutibacter oricola sp. Nov., a marine bacterium isolated from seawater

Abstract

A bacterial strain, UDC377T, was isolated from seawater samples collected at Seo-do on the coast of Dokdo island in the East Sea, and was subjected to taxonomic study using a polyphasic approach. Strain UDC377T was pale-yellow, Gram-staining-negative, non-motile, rod-shaped and aerobic. The strain grew optimally at 25-28°C, in the presence of 2% (w/v) NaCl and at pH 7.0-7.5. Strain UDC377T produced carotenoid pigments; however, it did not produce flexirubin-type pigments. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain UDC377T clustered with members of the genus Lutibacter and appeared most closely related to Lutibacter agarilyticus KYW566T (96.0% 16S rRNA gene sequence similarity) followed by L. aestuarii MA-My1T (95.0%), L. litoralis CL-TF09T (94.9%), L. maritimus S7-2T(94.1%) and L. flavus IMCC1507T (94.0%). The DNA G+C content of strain UDC377T was 30.8 mol%. Strain UDC377T contained MK-6 as the predominant menaquinone, iso-C15: 0 3-OH, iso-C15 : 0 and iso-C16 : 0 3-OH as the major fatty acids, and phosphatidylethanolamine, two unknown aminolipids and six unknown lipids as the major polar lipids. Based on phenotypic properties and phylogenetic data presented, strain UDC377T is considered to represent a nov.l sp.cies of the genus Lutibacter, for which the name Lutibacter oricola sp. nov. is proposed. The type strain is UDC377T (5DSM 24956T5KCTC 23668T).