Control of mammalian G protein signaling by N-terminal acetylation and the N-end rule pathway

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dc.contributor.authorS E Park-
dc.contributor.authorJ M Kim-
dc.contributor.authorO H Seok-
dc.contributor.authorH Cho-
dc.contributor.authorB Wadas-
dc.contributor.authorSeon-Young Kim-
dc.contributor.authorA Varshavsky-
dc.contributor.authorC S Hwang-
dc.date.accessioned2017-04-19T10:02:22Z-
dc.date.available2017-04-19T10:02:22Z-
dc.date.issued2015-
dc.identifier.issn0036-8075-
dc.identifier.uri10.1126/science.aaa3844ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12499-
dc.description.abstractRgs2, a regulator of G proteins, lowers blood pressure by decreasing signaling through Gαq. Human patients expressing Met-Leu-Rgs2 (ML-Rgs2) or Met-Arg-Rgs2 (MR-Rgs2) are hypertensive relative to people expressing wild-type Met-Gln-Rgs2 (MQ-Rgs2). We found that wild-type MQ-Rgs2 and its mutant, MR-Rgs2, were destroyed by the Ac/N-end rule pathway, which recognizes Nα-terminally acetylated (Nt-acetylated) proteins. The shortest-lived mutant, ML-Rgs2, was targeted by both the Ac/N-end rule and Arg/N-end rule pathways. The latter pathway recognizes unacetylated N-terminal residues. Thus, the Nt-acetylated Ac-MX-Rgs2 (X = Arg, Gln, Leu) proteins are specific substrates of the mammalian Ac/N-end rule pathway. Furthermore, the Ac/N-degron of Ac-MQ-Rgs2 was conditional, and Teb4, an endoplasmic reticulum (ER) membrane-embedded ubiquitin ligase, was able to regulate G protein signaling by targeting Ac-MX-Rgs2 proteins for degradation through their Nα-terminal acetyl group.-
dc.publisherAmer Assoc Advancement Science-
dc.titleControl of mammalian G protein signaling by N-terminal acetylation and the N-end rule pathway-
dc.title.alternativeControl of mammalian G protein signaling by N-terminal acetylation and the N-end rule pathway-
dc.typeArticle-
dc.citation.titleScience-
dc.citation.number6227-
dc.citation.endPage1252-
dc.citation.startPage1249-
dc.citation.volume347-
dc.contributor.affiliatedAuthorSeon-Young Kim-
dc.contributor.alternativeName박상은-
dc.contributor.alternativeName김정목-
dc.contributor.alternativeName석옥희-
dc.contributor.alternativeName조한나-
dc.contributor.alternativeNameWadas-
dc.contributor.alternativeName김선영-
dc.contributor.alternativeNameVarshavsky-
dc.contributor.alternativeName황철상-
dc.identifier.bibliographicCitationScience, vol. 347, no. 6227, pp. 1249-1252-
dc.identifier.doi10.1126/science.aaa3844-
dc.description.journalClassY-
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