Development of multiplex rt-PCR for simultaneous detection of garlic viruses and the incidence of garlic viral disease in garlic genetic resources

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dc.contributor.authorM Nam-
dc.contributor.authorY H Lee-
dc.contributor.authorC Y Park-
dc.contributor.authorM A Lee-
dc.contributor.authorY S Bae-
dc.contributor.authorSeung Mo Lim-
dc.contributor.authorJ H Lee-
dc.contributor.authorJae Sun Moon-
dc.contributor.authorS H Lee-
dc.date.accessioned2017-04-19T10:02:22Z-
dc.date.available2017-04-19T10:02:22Z-
dc.date.issued2015-
dc.identifier.issnI000-0113-
dc.identifier.uri10.5423/PPJ.NT.10.2014.0114ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12500-
dc.description.abstractGarlic generally becomes coinfected with several types of viruses belonging to the Potyvirus, Carlavirus, and Allexivirus genera. These viruses produce characteristically similar symptoms, they cannot be easily identified by electron microscopy (EM) or immunological detection methods, and they are currently widespread around the world, thereby affecting crop yields and crop quality adversely. For the early and reliable detection of garlic viruses, virus-specific sets of primers, including speciesspecific and genus-specific primers were designed. To effectively detect the twelve different types of garlic viruses, primer mixtures were tested and divided into two independent sets for multiplex polymerase chain reaction (PCR). The multiplex PCR assays were able to detect specific targets up to the similar dilution series with monoplex reverse transcription (RT)-PCR. Seventy-two field samples collected by the Gyeongbuk Agricultural Technology Administration were analyzed by multiplex RT-PCR. All seventy two samples were infected with at least one virus, and the coinfection rate was 78%. We conclude that the simultaneous detection system developed in this study can effectively detect and differentiate mixed viral infections in garlic.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleDevelopment of multiplex rt-PCR for simultaneous detection of garlic viruses and the incidence of garlic viral disease in garlic genetic resources-
dc.title.alternativeDevelopment of multiplex rt-PCR for simultaneous detection of garlic viruses and the incidence of garlic viral disease in garlic genetic resources-
dc.typeArticle-
dc.citation.titlePlant Pathology Journal-
dc.citation.number1-
dc.citation.endPage96-
dc.citation.startPage90-
dc.citation.volume31-
dc.contributor.affiliatedAuthorSeung Mo Lim-
dc.contributor.affiliatedAuthorJae Sun Moon-
dc.contributor.alternativeName남문-
dc.contributor.alternativeName이영훈-
dc.contributor.alternativeName박청열-
dc.contributor.alternativeName이민아-
dc.contributor.alternativeName배양수-
dc.contributor.alternativeName임승모-
dc.contributor.alternativeName이중환-
dc.contributor.alternativeName문제선-
dc.contributor.alternativeName이수헌-
dc.identifier.bibliographicCitationPlant Pathology Journal, vol. 31, no. 1, pp. 90-96-
dc.identifier.doi10.5423/PPJ.NT.10.2014.0114-
dc.subject.keywordGarlic-
dc.subject.keywordMultiplex RT-PCR-
dc.subject.keywordPlant virus-
dc.subject.keywordSimultaneous detection-
dc.subject.keywordVirus diagnosis-
dc.subject.localgarlic-
dc.subject.localGarlic-
dc.subject.localMultiplex RT-PCR-
dc.subject.localPlant virus-
dc.subject.localPlant viruses-
dc.subject.localplant virus-
dc.subject.localSimultaneous detection-
dc.subject.localsimultaneous detection-
dc.subject.localVirus diagnosis-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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