Putative endoglucanase PcGH5 from Phanerochaete chrysosporium is a beta-xylosidase that cleaves xylans in synergistic action with endo-xylanase

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dc.contributor.authorN D Huy-
dc.contributor.authorC L Nguyen-
dc.contributor.authorJeong-Woo Seo-
dc.contributor.authorD H Kim-
dc.contributor.authorS M Park-
dc.date.accessioned2017-04-19T10:02:23Z-
dc.date.available2017-04-19T10:02:23Z-
dc.date.issued2015-
dc.identifier.issn1389-1723-
dc.identifier.uri10.1016/j.jbiosc.2014.09.012ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12503-
dc.description.abstractA predicted endoglucanase gene (PcGH5) was cloned from Phanerochaete chysosporium, and expressed in Pichia pastoris. Although PcGH5 showed similarity with the conserved domains of a cellulase superfamily GH5, a β-glucosidase/6-phospho-β-glucosidase/β-galactosidase superfamily, and an endoglucanase, recombinant PcGH5 exhibited a β-xylosidase activity, rather than endoglucanase activity. Therefore, the predicted gene was named as PcXyl5. Further characterization of recombinant PcXyl5 showed not only catalysis of the hydrolysis of xylo-oligomers to xylose, but also displayed transglycosylation activity using alcohol as a receptor. Optimum pH of rPcXyl5 was found to be 5.5, whereas optimum temperature was 50°C. rPcXyl5 increased reducing sugar release of birchwood xylan, beechwood xylan, and arabinoxylan by 6.4%, 13%, 15.8%, respectively, in synergistic action with endo-xylanase. Interestingly, the late addition of rPcXyl5 into reaction with endo-xylanase resulted in a larger increase of reducing sugar release from pretreated barley straw that addition at the start or by treatment with endo-xylanases alone. The increases observed were 6.3% and 13.8%, respectively, showing a great potential application for hemicellulose saccharification.-
dc.publisherSoc Bioscience Bioengineering Japan-
dc.titlePutative endoglucanase PcGH5 from Phanerochaete chrysosporium is a beta-xylosidase that cleaves xylans in synergistic action with endo-xylanase-
dc.title.alternativePutative endoglucanase PcGH5 from Phanerochaete chrysosporium is a beta-xylosidase that cleaves xylans in synergistic action with endo-xylanase-
dc.typeArticle-
dc.citation.titleJournal of Bioscience and Bioengineering-
dc.citation.number4-
dc.citation.endPage420-
dc.citation.startPage416-
dc.citation.volume119-
dc.contributor.affiliatedAuthorJeong-Woo Seo-
dc.contributor.alternativeNameHuy-
dc.contributor.alternativeNameNguyen-
dc.contributor.alternativeName서정우-
dc.contributor.alternativeName김대혁-
dc.contributor.alternativeName박승문-
dc.identifier.bibliographicCitationJournal of Bioscience and Bioengineering, vol. 119, no. 4, pp. 416-420-
dc.identifier.doi10.1016/j.jbiosc.2014.09.012-
dc.subject.keywordGlycoside hydrolase family 5-
dc.subject.keywordHemicellulose-
dc.subject.keywordPhanerochaete chrysosporium-
dc.subject.keywordXylan-
dc.subject.keywordXylosidase-
dc.subject.localGlycoside hydrolase family 5-
dc.subject.localHemicellulose-
dc.subject.localPhanerochaete chrysosporium-
dc.subject.localphanerochaete chrysosporium-
dc.subject.localXylan-
dc.subject.localxylan-
dc.subject.localXylosidase-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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