DC Field | Value | Language |
---|---|---|
dc.contributor.author | N D Huy | - |
dc.contributor.author | C L Nguyen | - |
dc.contributor.author | Jeong-Woo Seo | - |
dc.contributor.author | D H Kim | - |
dc.contributor.author | S M Park | - |
dc.date.accessioned | 2017-04-19T10:02:23Z | - |
dc.date.available | 2017-04-19T10:02:23Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 1389-1723 | - |
dc.identifier.uri | 10.1016/j.jbiosc.2014.09.012 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/12503 | - |
dc.description.abstract | A predicted endoglucanase gene (PcGH5) was cloned from Phanerochaete chysosporium, and expressed in Pichia pastoris. Although PcGH5 showed similarity with the conserved domains of a cellulase superfamily GH5, a β-glucosidase/6-phospho-β-glucosidase/β-galactosidase superfamily, and an endoglucanase, recombinant PcGH5 exhibited a β-xylosidase activity, rather than endoglucanase activity. Therefore, the predicted gene was named as PcXyl5. Further characterization of recombinant PcXyl5 showed not only catalysis of the hydrolysis of xylo-oligomers to xylose, but also displayed transglycosylation activity using alcohol as a receptor. Optimum pH of rPcXyl5 was found to be 5.5, whereas optimum temperature was 50°C. rPcXyl5 increased reducing sugar release of birchwood xylan, beechwood xylan, and arabinoxylan by 6.4%, 13%, 15.8%, respectively, in synergistic action with endo-xylanase. Interestingly, the late addition of rPcXyl5 into reaction with endo-xylanase resulted in a larger increase of reducing sugar release from pretreated barley straw that addition at the start or by treatment with endo-xylanases alone. The increases observed were 6.3% and 13.8%, respectively, showing a great potential application for hemicellulose saccharification. | - |
dc.publisher | Soc Bioscience Bioengineering Japan | - |
dc.title | Putative endoglucanase PcGH5 from Phanerochaete chrysosporium is a beta-xylosidase that cleaves xylans in synergistic action with endo-xylanase | - |
dc.title.alternative | Putative endoglucanase PcGH5 from Phanerochaete chrysosporium is a beta-xylosidase that cleaves xylans in synergistic action with endo-xylanase | - |
dc.type | Article | - |
dc.citation.title | Journal of Bioscience and Bioengineering | - |
dc.citation.number | 4 | - |
dc.citation.endPage | 420 | - |
dc.citation.startPage | 416 | - |
dc.citation.volume | 119 | - |
dc.contributor.affiliatedAuthor | Jeong-Woo Seo | - |
dc.contributor.alternativeName | Huy | - |
dc.contributor.alternativeName | Nguyen | - |
dc.contributor.alternativeName | 서정우 | - |
dc.contributor.alternativeName | 김대혁 | - |
dc.contributor.alternativeName | 박승문 | - |
dc.identifier.bibliographicCitation | Journal of Bioscience and Bioengineering, vol. 119, no. 4, pp. 416-420 | - |
dc.identifier.doi | 10.1016/j.jbiosc.2014.09.012 | - |
dc.subject.keyword | Glycoside hydrolase family 5 | - |
dc.subject.keyword | Hemicellulose | - |
dc.subject.keyword | Phanerochaete chrysosporium | - |
dc.subject.keyword | Xylan | - |
dc.subject.keyword | Xylosidase | - |
dc.subject.local | Glycoside hydrolase family 5 | - |
dc.subject.local | Hemicellulose | - |
dc.subject.local | Phanerochaete chrysosporium | - |
dc.subject.local | phanerochaete chrysosporium | - |
dc.subject.local | Xylan | - |
dc.subject.local | xylan | - |
dc.subject.local | Xylosidase | - |
dc.description.journalClass | Y | - |
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