Optimizing promoters and secretory signal sequences for producing ethanol from inulin by recombinant Saccharomyces cerevisiae carrying Kluyveromyces marxianus inulinase = Kluyveromyces marxianus의 inulinase를 발현하는 재조합 효모에서 프로모터와 분비신호 조절을 통한 이눌린을 이용한 에탄올 생산

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Title
Optimizing promoters and secretory signal sequences for producing ethanol from inulin by recombinant Saccharomyces cerevisiae carrying Kluyveromyces marxianus inulinase = Kluyveromyces marxianus의 inulinase를 발현하는 재조합 효모에서 프로모터와 분비신호 조절을 통한 이눌린을 이용한 에탄올 생산
Author(s)
S J Hong; H J Kim; J W Kim; Dae-Hee Lee; J H Seo
Bibliographic Citation
Bioprocess and Biosystems Engineering, vol. 38, no. 2, pp. 263-272
Publication Year
2015
Abstract
Inulin is a polyfructan that is abundant in plants such as Jerusalem artichoke, chicory and dahlia. Inulinase can easily hydrolyze inulin to fructose, which is consumed by microorganisms. Generally, Saccharomyces cerevisiae, an industrial workhorse strain for bioethanol production, is known for not having inulinase activity. The inulinase gene from Kluyveromyces marxianus (KmINU), with the ability of converting inulin to fructose, was introduced into S. cerevisiae D452-2. The inulinase gene was fused to three different types of promoter (GPD, PGK1, truncated HXT7) and secretory signal sequence (KmINU, MFα1, SUC2) to generate nine expression cassettes. The inulin fermentation performance of the nine transformants containing different promoter and signal sequence combinations for inulinase production were compared to select an optimized expression system for efficient inulin fermentation. Among the nine inulinase-producing transformants, the S. cerevisiae carrying the PGK1 promoter and MFα1 signal sequence (S. cerevisiae D452-2/p426PM) showed not only the highest specific KmINU activity, but also the best inulin fermentation capability. Finally, a batch fermentation of the selected S. cerevisiae D452-2/p426PM in a bioreactor with 188.2 g/L inulin was performed to produce 80.2 g/L ethanol with 0.43 g ethanol/g inulin of ethanol yield and 1.22 g/L h of ethanol productivity.
Keyword
Consolidated bioprocessingEthanolInulinInulinaseSaccharomyces cerevisiae
ISSN
1615-7591
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s00449-014-1265-7
Type
Article
Appears in Collections:
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
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