Journal of Applied Biological Chemistry, vol. 58, no. 1, pp. 31-38
The rhizomes of Anemarrhena asphodeloide was extracted with 100% ethanol and concentrated subfractions were separated with medium pressure liquid chromatography-based activity profiling. One compound was isolated from the subfraction 10 through the repeated preparative high performance liquid chromatography (prep-HPLC). According to physico-chemical and spectroscopic data including NMR and MS, the chemical structures of the compound was determined as nyasol (1). Nyasol was exhibited potent inhibitory activity for NO (IC50: 12.5 μg/mL), tyrosinase (IC50: 12.5 μg/mL), melanin contents (IC50: 12.5 μg/mL), thymus and activation-regulated chemokine (TARC) production (IC50: 6.25 μg/mL). As a result, nyasol has an excellent inflammation-dependent anti-whitening and TARC production activity. It could be used to a large range of functional cosmetics.