Enhancing functional expression of heterologous proteins through random substitution of genetic codes in the 5' coding region

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Title
Enhancing functional expression of heterologous proteins through random substitution of genetic codes in the 5' coding region
Author(s)
Dae Eun Cheong; Kyong-Cheol KoYunjon Han; Ho Geun Jeon; Bong Hyun Sung; G J Kim; Jong Hyun ChoiJae Jun Song
Bibliographic Citation
Biotechnology and Bioengineering, vol. 112, no. 4, pp. 822-826
Publication Year
2015
Abstract
Recent studies using heterologous protein expression systems suggest that synonymous codons affect not only the expression but also the properties of the expressed protein. However, practical application of this information is challenging, and to date, efforts to employ bioinformatics tools to design synonymous codon mixes have been only marginally successful. Here, we sought to enhance the functional expression of heterologous protein in Escherichia coli through completely random substitution of the first ten codons with synonymous codons, using a previously isolated exocellulase CelEdx-SF301 as the model protein. Synonymous codon variants were generated by PCR using forward primers with mixed nucleotides at the third position in each codon and a conventional reverse primer. The resulting PCR products were inserted upstream of the fluorescent protein mCherry without linkers. After transformation and cultivation, colonies exhibiting red fluorescence were selected, and the activity of SF301-mCherry fusion proteins was tested. Synonymous codon variant fusion proteins exhibited 35- to 530-fold increases in functional expression compared with wild-type controls. Unlike results from other reports, we found that the stability of mRNA secondary structure in the 5' untranslated region and codon rarity were not correlated with functional expression level. Our work demonstrates that a completely random mixed of synonymous codons effectively enhances functional expression levels without the need for amino acid substitutions.
Keyword
Aggregation-prone proteinFunctional expressionSynonymous codonEscherichia coli
ISSN
0006-3592
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/bit.25478
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > Korea Preclinical Evaluation Center > 1. Journal Articles
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
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