Development and validation of a rapid immunochromatographic assay for detection of middle East respiratory syndrome coronavirus antigen in dromedary camels
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- Development and validation of a rapid immunochromatographic assay for detection of middle East respiratory syndrome coronavirus antigen in dromedary camels
- Dae Sub Song; G Ha; W Serhan; Y Eltahir; M Yusof; F Hashem; E Elsayed; B Marzoug; A Abdelazim; S A Muhairi
- Bibliographic Citation
- Journal of Clinical Microbiology, vol. 53, no. 4, pp. 1178-1182
- Publication Year
- We present here a rapid immunochromatographic assay for the detection of Middle East respiratory syndrome coronavirus (MERS-CoV) antigen in the nasal swabs of dromedary camels. The assay is based on the detection of MERS-CoV nucleocapsid protein in a short time frame using highly selective monoclonal antibodies at room temperature. The relative sensitivity and specificity of the assay were found to be 93.90% and 100%, respectively, compared to that of the UpE and open reading frame 1A (Orf1A) real-time reverse transcriptase PCR (RT-PCR). The results suggest that the assay developed here is a useful tool for the rapid diagnosis and epidemiological surveillance of MERS-CoV infection in dromedary camels.
- Amer Soc Microb
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- 1. Journal Articles > Journal Articles
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