Development and validation of a rapid immunochromatographic assay for detection of middle East respiratory syndrome coronavirus antigen in dromedary camels

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Title
Development and validation of a rapid immunochromatographic assay for detection of middle East respiratory syndrome coronavirus antigen in dromedary camels
Author(s)
Dae Sub Song; G Ha; W Serhan; Y Eltahir; M Yusof; F Hashem; E Elsayed; B Marzoug; A Abdelazim; S A Muhairi
Bibliographic Citation
Journal of Clinical Microbiology, vol. 53, no. 4, pp. 1178-1182
Publication Year
2015
Abstract
We present here a rapid immunochromatographic assay for the detection of Middle East respiratory syndrome coronavirus (MERS-CoV) antigen in the nasal swabs of dromedary camels. The assay is based on the detection of MERS-CoV nucleocapsid protein in a short time frame using highly selective monoclonal antibodies at room temperature. The relative sensitivity and specificity of the assay were found to be 93.90% and 100%, respectively, compared to that of the UpE and open reading frame 1A (Orf1A) real-time reverse transcriptase PCR (RT-PCR). The results suggest that the assay developed here is a useful tool for the rapid diagnosis and epidemiological surveillance of MERS-CoV infection in dromedary camels.
ISSN
0095-1137
Publisher
Amer Soc Microb
DOI
http://dx.doi.org/10.1128/JCM.03096-14
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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