Quick quantification of proteins by MALDI

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dc.contributor.authorS H Ahn-
dc.contributor.authorJ W Kang-
dc.contributor.authorJeong Hee Moon-
dc.contributor.authorK P Kim-
dc.contributor.authorS H Lee-
dc.contributor.authorM S Kim-
dc.date.accessioned2017-04-19T10:02:54Z-
dc.date.available2017-04-19T10:02:54Z-
dc.date.issued2015-
dc.identifier.issn10765174-
dc.identifier.uri10.1002/jms.3567ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12546-
dc.description.abstractPreviously, we reported that the matrix-assisted laser desorption ionization spectrum of a peptide became reproducible when an effective temperature was held constant. Using a calibration curve drawn by plotting the peptide-to-matrix ion abundance ratio versus the peptide concentration in a solid sample, a peptide could be quantified without the use of any internal standard. In this work, we quantified proteins by quantifying their tryptic peptides with the aforementioned method. We modified the digestion process; e.g. disulfide bonds were not cleaved, so that hardly any reagent other than trypsin remained after the digestion process. This allowed the preparation of a sample by the direct mixing of a digestion mixture with a matrix solution. We also observed that the efficiency of the matrix-to-peptide proton transfer, as measured by its reaction quotient, was similar for peptides with arginine at the C-terminus. With the reaction quotient averaged over many such peptides, we could rapidly quantify proteins. Most importantly, no peptide standard, not to mention its isotopically labeled analog, was needed in this method.-
dc.publisherWiley-
dc.titleQuick quantification of proteins by MALDI-
dc.title.alternativeQuick quantification of proteins by MALDI-
dc.typeArticle-
dc.citation.titleJournal of Mass Spectrometry-
dc.citation.number3-
dc.citation.endPage602-
dc.citation.startPage596-
dc.citation.volume50-
dc.contributor.affiliatedAuthorJeong Hee Moon-
dc.contributor.alternativeName안성희-
dc.contributor.alternativeName강정원-
dc.contributor.alternativeName문정희-
dc.contributor.alternativeName김광표-
dc.contributor.alternativeName이성훈-
dc.contributor.alternativeName김명수-
dc.identifier.bibliographicCitationJournal of Mass Spectrometry, vol. 50, no. 3, pp. 596-602-
dc.identifier.doi10.1002/jms.3567-
dc.subject.keywordMALDI-
dc.subject.keywordprotein quantificaton-
dc.subject.keywordquantification of hGH-
dc.subject.keywordquantification of myoglobin-
dc.subject.keywordtryptic digestion-
dc.subject.localMALDI-
dc.subject.localprotein quantificaton-
dc.subject.localquantification of hGH-
dc.subject.localquantification of myoglobin-
dc.subject.localtryptic digestion-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
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