Overexpression of the IbMYB1 gene in an orange-fleshed sweet potato cultivar produces a dual-pigmented transgenic sweet potato with improved antioxidant activity

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dc.contributor.authorSung-Chul Park-
dc.contributor.authorY H Kim-
dc.contributor.authorSun Ha Kim-
dc.contributor.authorYu Jeong Jeong-
dc.contributor.authorCha Young Kim-
dc.contributor.authorJ S Lee-
dc.contributor.authorJ Y Bae-
dc.contributor.authorM J Ahn-
dc.contributor.authorJae Cheol Jeong-
dc.contributor.authorHaeng Soon Lee-
dc.contributor.authorSang Soo Kwak-
dc.date.accessioned2017-04-19T10:03:03Z-
dc.date.available2017-04-19T10:03:03Z-
dc.date.issued2015-
dc.identifier.issn0031-9317-
dc.identifier.uri10.1111/ppl.12281ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12564-
dc.description.abstractThe R2R3-type protein IbMYB1 is a key regulator of anthocyanin biosynthesis in the storage roots of sweet potato [Ipomoea batatas (L.) Lam]. Previously, we demonstrated that IbMYB1 expression stimulated anthocyanin pigmentation in tobacco leaves and Arabidopsis. Here, we generated dual-pigmented transgenic sweet potato plants that accumulated high levels of both anthocyanins and carotenoids in a single sweet potato storage root. An orange-fleshed cultivar with high carotenoid levels was transformed with the IbMYB1 gene under the control of either the storage root-specific sporamin 1 (SPO1) promoter or the oxidative stress-inducible peroxidase anionic 2 (SWPA2) promoter. The SPO1-MYB transgenic lines exhibited higher anthocyanin levels in storage roots than empty vector control (EV) or SWPA2-MYB plants, but carotenoid content was unchanged. SWPA2-MYB transgenic lines exhibited higher levels of both anthocyanin and carotenoids than EV plants. Analysis of hydrolyzed anthocyanin extracts indicated that cyanidin and peonidin predominated in both overexpression lines. Quantitative reverse transcription-polymerase chain reaction analysis demonstrated that IbMYB1 expression in both IbMYB1 transgenic lines strongly induced the upregulation of several genes in the anthocyanin biosynthetic pathway, whereas the expression of carotenoid biosynthetic pathway genes varied between transgenic lines. Increased anthocyanin levels in transgenic plants also promoted the elevation of proanthocyanidin and total phenolic levels in fresh storage roots. Consequently, all IbMYB1 transgenic plants displayed much higher antioxidant activities than EV plants. In field cultivations, storage root yields varied between the transgenic lines. Taken together, our results indicate that overexpression of IbMYB1 is a highly promising strategy for the generation of transgenic plants with enhanced antioxidant capacity.-
dc.publisherWiley-
dc.titleOverexpression of the IbMYB1 gene in an orange-fleshed sweet potato cultivar produces a dual-pigmented transgenic sweet potato with improved antioxidant activity-
dc.title.alternativeOverexpression of the IbMYB1 gene in an orange-fleshed sweet potato cultivar produces a dual-pigmented transgenic sweet potato with improved antioxidant activity-
dc.typeArticle-
dc.citation.titlePhysiologia Plantarum-
dc.citation.number4-
dc.citation.endPage537-
dc.citation.startPage525-
dc.citation.volume153-
dc.contributor.affiliatedAuthorSung-Chul Park-
dc.contributor.affiliatedAuthorSun Ha Kim-
dc.contributor.affiliatedAuthorYu Jeong Jeong-
dc.contributor.affiliatedAuthorCha Young Kim-
dc.contributor.affiliatedAuthorJae Cheol Jeong-
dc.contributor.affiliatedAuthorHaeng Soon Lee-
dc.contributor.affiliatedAuthorSang Soo Kwak-
dc.contributor.alternativeName박성철-
dc.contributor.alternativeName김윤희-
dc.contributor.alternativeName김선하-
dc.contributor.alternativeName정유정-
dc.contributor.alternativeName김차영-
dc.contributor.alternativeName이준설-
dc.contributor.alternativeName배지영-
dc.contributor.alternativeName안미정-
dc.contributor.alternativeName정재철-
dc.contributor.alternativeName이행순-
dc.contributor.alternativeName곽상수-
dc.identifier.bibliographicCitationPhysiologia Plantarum, vol. 153, no. 4, pp. 525-537-
dc.identifier.doi10.1111/ppl.12281-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Jeonbuk Branch Institute > 1. Journal Articles
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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