TAGLN2 regulates T cell activation by stabilizing the actin cytoskeleton at the immunological synapse

Cited 43 time in scopus
Metadata Downloads
Title
TAGLN2 regulates T cell activation by stabilizing the actin cytoskeleton at the immunological synapse
Author(s)
B R Na; H R Kim; I Piragyte; Hyun-Mee Oh; M S Kwon; U Akber; H S Lee; D S Park; W K Song; Z Y Park; S H Im; Mun Chual Rho; Y M Hyun; M Kim; C D Jun
Bibliographic Citation
Journal of Cell Biology, vol. 209, no. 1, pp. 143-162
Publication Year
2015
Abstract
The formation of an immunological synapse (IS) requires tight regulation of actin dynamics by many actin polymerizing/depolymerizing proteins. However, the significance of actin stabilization at the IS remains largely unknown. In this paper, we identify a novel function of TAGLN2-an actin-binding protein predominantly expressed in T cells-in stabilizing cortical F-actin, thereby maintaining F-actin contents at the IS and acquiring LFA-1 (leukocyte function-associated antigen-1) activation after T cell receptor stimulation. TAGLN2 blocks actin depolymerization and competes with cofilin both in vitro and in vivo. Knockout of TAGLN2 (TAGLN2-/-) reduced F-actin content and destabilized F-actin ring formation, resulting in decreased cell adhesion and spreading. TAGLN2-/-T cells displayed weakened cytokine production and cytotoxic effector function. These findings reveal a novel function of TAGLN2 in enhancing T cell responses by controlling actin stability at the IS.
ISSN
0021-9525
Publisher
Rockefeller Univ Press
DOI
http://dx.doi.org/10.1083/jcb.201407130
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.